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dc.contributor.authorCosta, Tiago R. D.
dc.contributor.authorlangovan, Aravindan I
dc.contributor.authorUkleja, Marta
dc.contributor.authorRedjez, Adam
dc.contributor.authorSantini, Joanne M.
dc.contributor.authorSmith, Terry K.
dc.contributor.authorEgelman, Edward H.
dc.contributor.authorWaksman, Gabriel
dc.date.accessioned2016-09-09T10:30:10Z
dc.date.available2016-09-09T10:30:10Z
dc.date.issued2016-09-08
dc.identifier.citationCosta , T R D , langovan , A I , Ukleja , M , Redjez , A , Santini , J M , Smith , T K , Egelman , E H & Waksman , G 2016 , ' Structure of the bacterial sex F pilus reveals an assembly of a stoichiometric protein-phospholipid complex ' , Cell , vol. 166 , no. 6 , e10 , pp. 1436-1444 . https://doi.org/10.1016/j.cell.2016.08.025en
dc.identifier.issn0092-8674
dc.identifier.otherPURE: 244482702
dc.identifier.otherPURE UUID: 395bf38f-3e54-490a-94b3-b779ac838f2b
dc.identifier.otherScopus: 84986294152
dc.identifier.otherWOS: 000386339900016
dc.identifier.urihttp://hdl.handle.net/10023/9465
dc.descriptionThis work was supported by Wellcome Trust grants 098302 to G.W. and 093228 to T.K.S. and NIH grant GM035269 to E.H.E.en
dc.description.abstractConjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F “sex” pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 Å resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection.
dc.format.extent20
dc.language.isoeng
dc.relation.ispartofCellen
dc.rightsCopyright: 2016 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).en
dc.subjectPilusen
dc.subjectConjugationen
dc.subjectType 4 Secretion Systemen
dc.subjectPhospholipiden
dc.subjectProtein-phospholipid complexen
dc.subjectStructural biologyen
dc.subjectCryoelectron Microscopyen
dc.subjectQH301 Biologyen
dc.subjectDASen
dc.subject.lccQH301en
dc.titleStructure of the bacterial sex F pilus reveals an assembly of a stoichiometric protein-phospholipid complexen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1016/j.cell.2016.08.025
dc.description.statusPeer revieweden


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