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dc.contributor.authorCox, Martin
dc.contributor.authorKawaguchu, So
dc.contributor.authorKing, Robert
dc.contributor.authorDholakia, Kishan
dc.contributor.authorBrown, C Tom A
dc.date.accessioned2016-08-25T23:34:43Z
dc.date.available2016-08-25T23:34:43Z
dc.date.issued2015
dc.identifier.citationCox , M , Kawaguchu , S , King , R , Dholakia , K & Brown , C T A 2015 , ' Internal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniques ' , Marine and Freshwater Behaviour and Physiology , vol. In press . https://doi.org/10.1080/10236244.2015.1073455en
dc.identifier.issn1023-6244
dc.identifier.otherPURE: 201549524
dc.identifier.otherPURE UUID: 4701e3fb-2832-4f5c-94c7-d5cb70e3eea5
dc.identifier.otherScopus: 84945458265
dc.identifier.otherWOS: 000363017900001
dc.identifier.urihttp://hdl.handle.net/10023/9376
dc.descriptionMJC is funded by an Australian Research Council grant FS110200057. CTAB acknowledges support for instrument development and shipping costs from the United Kingdom Engineering and Physical Sciences Research Council Grant EP/M000869/1 (Shaped Light at the Interface).en
dc.description.abstractThe accurate observation of physiological changes on in vivo samples of important animal species such as Euphasia superba (Antarctic krill) is an important goal in helping to understand how environmental changes can affect animal development. Using a custom made ‘krill trap’, live un-anaesthetized krill were confined for seven hours, during which three hours of optical imaging were obtained and no subsequent ill effects observed. The trap enabled two imaging methods to be employed: Optical Coherence Tomography (OCT) and microscopy. OCT enabled internal structure and tissues to be imaged to a depth of approximately 2 mm and resolution of approximately 12 μm. Microscopy was used to observe heart rate. During our experiments, we imaged a range of internal structures in live animals including the heart and gastric areas. The trap design enables a new generation of mixed modality imaging of these animals in vivo. These techniques will enable detailed studies of the internal physiology of live krill to be undertaken under a wide range of environmental conditions and have the potential to highlight important variations in behaviour and animal development.
dc.language.isoeng
dc.relation.ispartofMarine and Freshwater Behaviour and Physiologyen
dc.rights© 2015 Taylor & Francis. This is an Accepted Manuscript of an article published by Taylor & Francis in Marine and Freshwater Behaviour and Physiology on 25/08/2015, available online: http://wwww.tandfonline.com/10.1080/10236244.2015.1073455en
dc.subjectKrill trapen
dc.subjectLive animalen
dc.subjectMorphological observationsen
dc.subjectOptical Coherence Tomographyen
dc.subjectOptical imagingen
dc.subjectQC Physicsen
dc.subjectQL Zoologyen
dc.subjectDASen
dc.subject.lccQCen
dc.subject.lccQLen
dc.titleInternal physiology of live krill revealed using new aquaria techniques and mixed optical microscopy and optical coherence tomography (OCT) imaging techniquesen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1080/10236244.2015.1073455
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-08-25
dc.identifier.urlhttp://www.tandfonline.com/doi/full/10.1080/10236244.2015.1073455#_i19en


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