Old drugs to treat resistant bugs : methicillin-resistant Staphylococcus aureus isolates with mecC are susceptible to a combination of penicillin and clavulanic acid
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β-Lactam resistance in methicillin-resistant Staphylococcus aureus (MRSA) is mediated by the expression of an alternative penicillin-binding protein 2a (PBP2a) (encoded by mecA) with a low affinity for β-lactam antibiotics. Recently, a novel variant of mecA, known as mecC, was identified in MRSA isolates from both humans and animals. In this study, we demonstrate that mecC-encoded PBP2c does not mediate resistance to penicillin. Rather, broad-spectrum β-lactam resistance in MRSA strains carrying mecC (mecC-MRSA strains) is mediated by a combination of both PBP2c and the distinct β-lactamase encoded by the blaZ gene of strain LGA251 (blaZLGA251), which is part of mecC-encoding staphylococcal cassette chromosome mec (SCCmec) type XI. We further demonstrate that mecC-MRSA strains are susceptible to the combination of penicillin and the β-lactam inhibitor clavulanic acid in vitro and that the same combination is effective in vivo for the treatment of experimental mecC-MRSA infection in wax moth larvae. Thus, we demonstrate how the distinct biological differences between mecA- and mecC-encoded PBP2a and PBP2c have the potential to be exploited as a novel approach for the treatment of mecC-MRSA infections.
Ba , X , Harrison , E M , Lovering , A L , Gleadall , N , Zadoks , R , Parkhill , J , Peacock , S J , Holden , M T G , Paterson , G K & Holmes , M A 2015 , ' Old drugs to treat resistant bugs : methicillin-resistant Staphylococcus aureus isolates with mecC are susceptible to a combination of penicillin and clavulanic acid ' , Antimicrobial Agents and Chemotherapy , vol. 59 , no. 12 , pp. 7396-7404 . https://doi.org/10.1128/AAC.01469-15
Antimicrobial Agents and Chemotherapy
© 2016, American Society for Microbiology. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at aac.asm.org / https://dx.doi.org/10.1128/AAC.01469-15
DescriptionThis work was supported by a Medical Research Council (MRC) partnership grant (G1001787/1) held between the Department of Veterinary Medicine, University of Cambridge (M.A.H.), the School of Clinical Medicine, University of Cambridge (S.J.P.), the Moredun Research Institute (R.Z.), and the Wellcome Trust Sanger Institute (J.P. and S.J.P.).
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