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dc.contributor.authorVarnaitė , Renata
dc.contributor.authorMacNeill, Stuart Andrew
dc.identifier.citationVarnaitė , R & MacNeill , S A 2016 , ' Meet the neighbours: mapping local protein interactomes by proximity-dependent labelling with BioID ' , Proteomics , vol. 16 , no. 19 , pp. 2503-2518 .
dc.identifier.otherPURE: 243453561
dc.identifier.otherPURE UUID: 6d894040-86c2-44ed-b89f-477a97a99ada
dc.identifier.otherScopus: 84989932279
dc.identifier.otherWOS: 000385814700001
dc.description.abstractProximity-dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a protein of interest in living cells. BioID relies on fusion of the protein of interest with a mutant form of the biotin ligase enzyme BirA (BirA*) that is capable of promiscuously biotinylating proximal proteins irrespective of whether these interact directly or indirectly with the fusion protein or are merely located in the same sub-cellular neighbourhood. The covalent addition of biotin allows the labelled proteins to be purified from cell extracts on the basis of their affinity for streptavidin and identified by mass spectrometry. To date, BioID has been successfully applied to study a variety of proteins and processes in mammalian cells and unicellular eukaryotes and has been shown to be particularly suited to the study of insoluble or inaccessible cellular structures and for detecting weak or transient protein associations. Here, we provide an introduction to BioID, together with a detailed summary of where and how the method has been applied to date, and briefly discuss technical aspects involved in the planning and execution of a BioID study.
dc.rights© 2016 The Authors. Proteomics Published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectProtein-protein interactionsen
dc.subjectProximity dependent biotin identification (BioID)en
dc.subjectPromiscuous biotin ligase (BirA*)en
dc.subjectQH301 Biologyen
dc.titleMeet the neighbours: mapping local protein interactomes by proximity-dependent labelling with BioIDen
dc.typeJournal itemen
dc.contributor.sponsorMedical Research Councilen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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