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dc.contributor.authorCheah, Menghon
dc.contributor.authorAndrews, Melissa Renee
dc.contributor.authorChew, Daniel
dc.contributor.authorMoloney, Elizabeth
dc.contributor.authorVerhaagen, Joost
dc.contributor.authorFassler, Reinhard
dc.contributor.authorFawcett, James
dc.date.accessioned2016-07-12T11:30:12Z
dc.date.available2016-07-12T11:30:12Z
dc.date.issued2016-07-06
dc.identifier.citationCheah , M , Andrews , M R , Chew , D , Moloney , E , Verhaagen , J , Fassler , R & Fawcett , J 2016 , ' Expression of an activated integrin promotes long-distance sensory axon regeneration in the spinal cord ' The Journal of Neuroscience , vol. 36 , no. 27 , pp. 7283-7297 . https://doi.org/10.1523/JNEUROSCI.0901-16.2016en
dc.identifier.issn0270-6474
dc.identifier.otherPURE: 243606485
dc.identifier.otherPURE UUID: 6f7403bd-f15a-4174-b201-4b8b27530057
dc.identifier.otherScopus: 84977543684
dc.identifier.otherWOS: 000379021800018
dc.identifier.urihttp://hdl.handle.net/10023/9117
dc.descriptionThis work was supported by grants from the Christopher and Dana Reeve Foundation, the Medical Research Council, the European Research Council ECMneuro, and the Cambridge National Health and Medical Research Council Biomedical Research Centre.en
dc.description.abstractAfter CNS injury, axon regeneration is blocked by an inhibitory environment consisting of the highly upregulated tenascin-C and chondroitin sulfate proteoglycans (CSPGs). Tenascin-C promotes growth of axons if they express a tenascin-binding integrin, particularly α9β1. Additionally, integrins can be inactivated by CSPGs, and this inhibition can be overcome by the presence of a β1-binding integrin activator, kindlin-1. We examined the synergistic effect of α9 integrin and kindlin-1 on sensory axon regeneration in adult rat spinal cord after dorsal root crush and adeno-associated virus transgene expression in dorsal root ganglia. After 12 weeks, axons from C6–C7 dorsal root ganglia regenerated through the tenascin-C-rich dorsal root entry zone into the dorsal column up to C1 level and above (25mm axon length) through a normal pathway. Animals also showed anatomical and electrophysiological evidence of reconnection to the dorsal horn and behavioral recovery in mechanical pressure, thermal pain, and ladder-walking tasks. Expression of α9 integrin or kindlin-1 alone promoted much less regeneration and recovery.
dc.format.extent15
dc.language.isoeng
dc.relation.ispartofThe Journal of Neuroscienceen
dc.rightsCopyright © 2016 the Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution License Creative Commons Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.en
dc.subjectAdeno-associated virusen
dc.subjectAlpha9 integrinen
dc.subjectAxon regenerationen
dc.subjectDorsal root ganglionen
dc.subjectKindlin-1en
dc.subjectSpinal corden
dc.subjectRC0321 Neuroscience. Biological psychiatry. Neuropsychiatryen
dc.subjectNeuroscience(all)en
dc.subjectNDASen
dc.subject.lccRC0321en
dc.titleExpression of an activated integrin promotes long-distance sensory axon regeneration in the spinal corden
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.identifier.doihttps://doi.org/10.1523/JNEUROSCI.0901-16.2016
dc.description.statusPeer revieweden


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