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Biochemical and genetic analysis of the role of the viral polymerase in enterovirus recombination

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Nucl._Acids_Res._2016_Woodman_nar_gkw567.pdf (2.849Mb)
Date
19/08/2016
Author
Woodman, Andrew
Arnold, Jamie
Cameron, Craig
Evans, David John
Funder
BBSRC
Grant ID
BB/M009343/1
Keywords
Pollovirus
Picornavirus
Polymerase
Fidelity
Recombination
Evolution
QD Chemistry
QH301 Biology
NDAS
BDC
SDG 3 - Good Health and Well-being
Metadata
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Abstract
Genetic recombination in single-strand, positive-sense RNA viruses is a poorly understand mechanism responsible for generating extensive genetic change and novel phenotypes. By moving a critical cis-acting replication element (CRE) from the polyprotein coding region to the 3’ non-coding region we have further developed a cell-based assay (the 3’CRE-REP assay) to yield recombinants throughout the non-structural coding region of poliovirus from dually transfected cells. We have additionally developed a defined biochemical assay in which the only protein present is the poliovirus RNA dependent RNA polymerase (RdRp), which recapitulates the strand transfer events of the recombination process. We have used both assays to investigate the role of the polymerase fidelity and nucleotide turnover rates in recombination. Our results, of both poliovirus intertypic and intratypic recombination in the CRE-REP assay and using a range of polymerase variants in the biochemical assay, demonstrate that RdRp fidelity is a fundamental determinant of recombination frequency. High fidelity polymerases exhibit reduced recombination and low fidelity polymerases exhibit increased recombination in both assays. These studies provide the basis for the analysis of poliovirus recombination throughout the non-structural region of the virus genome and provide a defined biochemical assay to further dissect this important evolutionary process.
Citation
Woodman , A , Arnold , J , Cameron , C & Evans , D J 2016 , ' Biochemical and genetic analysis of the role of the viral polymerase in enterovirus recombination ' , Nucleic Acids Research , vol. 44 , no. 14 , pp. 6883-6895 . https://doi.org/10.1093/nar/gkw567
Publication
Nucleic Acids Research
Status
Peer reviewed
DOI
https://doi.org/10.1093/nar/gkw567
ISSN
0305-1048
Type
Journal article
Rights
© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Description
Funding: Biotechnology and Biological Sciences Research Council [BB/M009343/1] and PhD. studentship funding for AW (to D.J.E.); NIH [R01 AI045818 from NIAID to C.E.C.). Funding for open access charge: Biotechnology and Biological Sciences Research Council and University of St. Andrews.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/9050

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