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dc.contributor.authorPaul, Shubhajit
dc.contributor.authorBode, Bela Ernest
dc.contributor.authorMatysik, Jörg
dc.contributor.authorAlia, Alia
dc.date.accessioned2016-06-24T23:30:59Z
dc.date.available2016-06-24T23:30:59Z
dc.date.issued2015-10-29
dc.identifier198032024
dc.identifier763e8e7b-df6c-4f21-8e14-58867a25cfbc
dc.identifier84946036095
dc.identifier000363994000048
dc.identifier.citationPaul , S , Bode , B E , Matysik , J & Alia , A 2015 , ' Photochemically induced dynamic nuclear polarization observed by solid-state NMR in a uniformly 13 C-isotope labeled photosynthetic reaction center ' , Journal of Physical Chemistry B , vol. 119 , no. 43 , pp. 13897-13903 . https://doi.org/10.1021/acs.jpcb.5b04542en
dc.identifier.issn1520-6106
dc.identifier.otherORCID: /0000-0002-3384-271X/work/27582725
dc.identifier.urihttps://hdl.handle.net/10023/9046
dc.descriptionFinancial support from the Netherlands Organisation for Scientific Research (NWO, 818.02.019) and the Deutsche Forschungsgemeinschaft (DFG, MA 497/2-1) is gratefully acknowledged.en
dc.description.abstractA sample of solubilized and quinone-depleted reaction centers (RC) from the purple bacterium Rhodobacter (R.) sphaeroides wild-type (WT) has been prepared entirely 13C and 15N isotope labelled at all positions of the protein as well as of the cofactors. In this sample, the occurrence of the solid-state photo-CIDNP (photochemically induced dynamic nuclear polarization) effect has been probed by 13C solid-state magic-angle spinning (MAS) NMR under illumination. Under continuous illumination, signal intensities are modified by the three-spin mixing (TSM) mechanism. Time-resolved illumination experiments reveal the occurrence of light-induced nuclear polarization on the time-scale of hundreds of microseconds, initially dominated by the transient polarization of the singlet branch of the radical-pair mechanism (RPM). A first kinetic analysis shows that the lifetime of the polarization from the singlet branch, indicated by the enhanced absorptive intensities of the signals from aliphatic carbons, is significantly extended. Upon arrival of the polarization from the triplet decay branch, emissive polarization caused by the three-spin mixing mechanism (TSM) is observed. Also this arrival is significantly delayed. The decay of TSM polarization occurs in two steps, assigned to intra- and intermolecular spin diffusion.
dc.format.extent804359
dc.language.isoeng
dc.relation.ispartofJournal of Physical Chemistry Ben
dc.subjectPhotosynthesisen
dc.subjectElectron transferen
dc.subjectNuclear polarizationen
dc.subjectSpin diffusionen
dc.subjectQD Chemistryen
dc.subjectNDASen
dc.subject.lccQDen
dc.titlePhotochemically induced dynamic nuclear polarization observed by solid-state NMR in a uniformly 13C-isotope labeled photosynthetic reaction centeren
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.contributor.institutionUniversity of St Andrews. Centre of Magnetic Resonanceen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. EaSTCHEMen
dc.identifier.doi10.1021/acs.jpcb.5b04542
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-06-25


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