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dc.contributor.authorBallard, E
dc.contributor.authorCoote, Peter John
dc.identifier.citationBallard , E & Coote , P J 2016 , ' Enhancement of antibiotic efficacy against multi-drug resistant Pseudomonas aeruginosa infections via combination with curcumin and 1-(1-Naphthylmethyl)-Piperazine ' , Journal of Antimicrobial Agents , vol. 2 , no. 2 , 1000116 .
dc.identifier.otherPURE: 242704485
dc.identifier.otherPURE UUID: 4dbabdb9-ead5-424f-be68-9569391f6fa4
dc.identifier.otherORCID: /0000-0001-5190-805X/work/40963220
dc.description.abstractObjective: The aim of this study was to determine if the plant phenolic curcumin (CUR) and the arylpiperazine 1-(1-naphthylmethyl)-piperazine (NMP) could restore antibiotic efficacy versus MDR P. aeruginosa infection. Methods: The MICs of piperacillin, meropenem and levofloxacin in the presence or absence of CUR or NMP against a MDR strain that over-expresses the MexAB-OprM efflux-pump and the isogenic parent strain were compared. The efficacy of the same combination treatments was also tested in a Galleria mellonella in vivo infection model and larval survival and bacterial burden compared. Results: In vitro, CUR restored the activity of piperacillin, meropenem and levofloxacin versus the MDR strain of P. aeruginosa only weakly. There was no evidence in vitro of a similar effect with NMP. In vivo, treatment of G. mellonella larvae infected with the MDR strain with a combination of NMP or CUR plus levofloxacin, and piperacillin plus CUR, resulted in enhanced therapeutic benefit compared to the monotherapies. When compared with monotherapies, the enhanced efficacy of the combination treatments correlated with reduced bacterial burden. Conclusion: CUR and NMP restored the efficacy of antibiotic therapy in vivo versus MDR P. aeruginosa infection.
dc.relation.ispartofJournal of Antimicrobial Agentsen
dc.rights© 2016 Ballard E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectPseudomonas aeruginosaen
dc.subjectEfflux pump inhibitorsen
dc.subjectGalleria mellonellaen
dc.subjectQH301 Biologyen
dc.subjectQR Microbiologyen
dc.titleEnhancement of antibiotic efficacy against multi-drug resistant Pseudomonas aeruginosa infections via combination with curcumin and 1-(1-Naphthylmethyl)-Piperazineen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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