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dc.contributor.authorRoulston, Claire
dc.contributor.authorLuke, Garry Alec
dc.contributor.authorde Felipe, Pablo
dc.contributor.authorRuan, Lin
dc.contributor.authorCope, Jonathan
dc.contributor.authorNicholson, John
dc.contributor.authorSukhodub, Andriy
dc.contributor.authorTilsner, Jens
dc.contributor.authorRyan, Martin Denis
dc.date.accessioned2016-06-03T08:30:10Z
dc.date.available2016-06-03T08:30:10Z
dc.date.issued2016-08
dc.identifier.citationRoulston , C , Luke , G A , de Felipe , P , Ruan , L , Cope , J , Nicholson , J , Sukhodub , A , Tilsner , J & Ryan , M D 2016 , ' "2A-like" signal sequences mediating translational recoding : a novel form of dual protein targeting ' Traffic , vol. 17 , no. 8 , pp. 923-939 . https://doi.org/10.1111/tra.12411en
dc.identifier.issn1398-9219
dc.identifier.otherPURE: 242479277
dc.identifier.otherPURE UUID: 29517e88-80d2-475c-baf1-442fbadcb1f3
dc.identifier.otherScopus: 84978388646
dc.identifier.otherORCID: /0000-0002-0012-0614/work/47136045
dc.identifier.urihttp://hdl.handle.net/10023/8924
dc.descriptionThe authors gratefully acknowledge the support of the UK Biotechnology and Biological Sciences Research Council (BBSRC) who funded this research. The authors also gratefully acknowledge the support of the Wellcome Trust for the provision of mass spectrometry facilities at St Andrews.en
dc.description.abstractWe report the initial characterisation of an N-terminal oligopeptide ‘2A-like’ sequence that is able to function both as a signal sequence and as a translational recoding element. Due to this translational recoding activity, two forms of nascent polypeptide are synthesised: (i) when 2A-mediated translational recoding has not occurred: the nascent polypeptide is fused to the 2A-like N-terminal signal sequence and the fusion translation product is targeted to the exocytic pathway, and, (ii) a translation product where 2A-mediated translational recoding has occurred: the 2A-like signal sequence is synthesised as a separate translation product and, therefore, the nascent (downstream) polypeptide lacks the 2A-like signal sequence and is localised to the cytoplasm. This type of dual-functional signal sequence results, therefore, in the partitioning of the translation products between the two sub-cellular sites and represents a newly described form of dual protein targeting.
dc.format.extent17
dc.language.isoeng
dc.relation.ispartofTrafficen
dc.rights© 2016 The Authors. Traffic published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.subjectTranslational recodingen
dc.subject2Aen
dc.subjectSignal sequenceen
dc.subjectSecretory pathwayen
dc.subjectDual protein targetingen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.title"2A-like" signal sequences mediating translational recoding : a novel form of dual protein targetingen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1111/tra.12411
dc.description.statusPeer revieweden


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