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The use of the Rx spin label in orientation measurement on proteins, by EPR

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dc.contributor.authorStevens, M A
dc.contributor.authorMcKay, Johannes Erik
dc.contributor.authorRobinson, J L S
dc.contributor.authorEl Mkami, Hassane
dc.contributor.authorSmith, Graham Murray
dc.contributor.authorNorman, D G
dc.date.accessioned2016-05-17T16:30:03Z
dc.date.available2016-05-17T16:30:03Z
dc.date.issued2016-02-28
dc.identifier.citationStevens , M A , McKay , J E , Robinson , J L S , El Mkami , H , Smith , G M & Norman , D G 2016 , ' The use of the Rx spin label in orientation measurement on proteins, by EPR ' , Physical Chemistry Chemical Physics , vol. 18 , no. 8 , pp. 5799-5806 . https://doi.org/10.1039/c5cp04753fen
dc.identifier.issn1463-9076
dc.identifier.otherPURE: 231916113
dc.identifier.otherPURE UUID: ae36211e-9722-410a-936c-99d65148b42a
dc.identifier.otherPubMed: 26426572
dc.identifier.otherScopus: 84958999274
dc.identifier.otherORCID: /0000-0001-6578-7412/work/59464780
dc.identifier.otherORCID: /0000-0002-0552-5784/work/60195406
dc.identifier.otherWOS: 000371953000012
dc.identifier.urihttp://hdl.handle.net/10023/8818
dc.descriptionM.A.S. & J.E.M. would like to acknowledge funding from the EPSRC as part of the iMR-CDT. The Authors would like to acknowledge funding from The MRC UK, Grant G1100021, EPSRC Basic Technology EP/F039034/1, and from the Wellcome Trust 099149/Z/12/Z.en
dc.description.abstractThe bipedal spin label Rx is more restricted in its conformation and dynamics than its monopodal counterpart R1. To systematically investigate the utility of the Rx label, we have attempted to comprehensively survey the attachment of Rx to protein secondary structures. We have examined the formation, structure and dynamics of the spin label in relation to the underlying protein in order to determine feasibility and optimum conditions for distance and orientation measurement by pulsed EPR. The labeled proteins have been studied using molecular dynamics, CW EPR, pulsed EPR distance measurement at X-band and orientation measurement at W-band. The utility of different modes and positions of attachment have been compared and contrasted.
dc.language.isoeng
dc.relation.ispartofPhysical Chemistry Chemical Physicsen
dc.rightsCopyright 2015 the Authors. This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence (http://creativecommons.org/licenses/by-nc/3.0/).en
dc.subjectQC Physicsen
dc.subjectQD Chemistryen
dc.subjectNDASen
dc.subject.lccQCen
dc.subject.lccQDen
dc.titleThe use of the Rx spin label in orientation measurement on proteins, by EPRen
dc.typeJournal articleen
dc.contributor.sponsorThe Wellcome Trusten
dc.contributor.sponsorEPSRCen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.identifier.doihttps://doi.org/10.1039/c5cp04753f
dc.description.statusPeer revieweden
dc.identifier.urlhttp://www.rsc.org/suppdata/c5/cp/c5cp04753f/c5cp04753f1.pdfen
dc.identifier.grantnumber099149/Z/12/Zen
dc.identifier.grantnumberEP/F039034/1en


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