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Nanoparticle suspensions enclosed in methylcellulose : a new approach for quantifying nanoparticles in transmission electron microscopy
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dc.contributor.author | Hacker, Christian | |
dc.contributor.author | Asadi, Jalal | |
dc.contributor.author | Pliotas, Christos | |
dc.contributor.author | Ferguson, Sophie Grace Alicia | |
dc.contributor.author | Sherry, Lee | |
dc.contributor.author | Marius, Phedra | |
dc.contributor.author | Tello, Javier | |
dc.contributor.author | Jackson, David | |
dc.contributor.author | Naismith, James Henderson | |
dc.contributor.author | Lucocq, John Milton | |
dc.date.accessioned | 2016-05-12T12:30:04Z | |
dc.date.available | 2016-05-12T12:30:04Z | |
dc.date.issued | 2016-05-04 | |
dc.identifier | 241943444 | |
dc.identifier | fddadfcf-c62b-4c65-9505-8c50a1f44c77 | |
dc.identifier | 84965138214 | |
dc.identifier | 000375428500002 | |
dc.identifier.citation | Hacker , C , Asadi , J , Pliotas , C , Ferguson , S G A , Sherry , L , Marius , P , Tello , J , Jackson , D , Naismith , J H & Lucocq , J M 2016 , ' Nanoparticle suspensions enclosed in methylcellulose : a new approach for quantifying nanoparticles in transmission electron microscopy ' , Scientific Reports , vol. 6 , 25275 . https://doi.org/10.1038/srep25275 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.other | ORCID: /0000-0002-4309-4858/work/31524144 | |
dc.identifier.other | ORCID: /0000-0001-6637-2155/work/64034496 | |
dc.identifier.other | ORCID: /0000-0002-5191-0093/work/64361161 | |
dc.identifier.uri | https://hdl.handle.net/10023/8790 | |
dc.description | This work was supported by the University of St Andrews and Nanomorphomics group funds. The work forms part of an International Patent Application No. PCT/GB2015/052482. | en |
dc.description.abstract | Nanoparticles are of increasing importance in biomedicine but quantification is problematic because current methods depend on indirect measurements at low resolution. Here we describe a new high-resolution method for measuring and quantifying nanoparticles in suspension. It involves premixing nanoparticles in a hydrophilic support medium (methylcellulose) before introducing heavy metal stains for visualization in small air-dried droplets by transmission electron microscopy (TEM). The use of methylcellulose avoids artifacts of conventional negative stain-TEM by (1) restricting interactions between the nanoparticles, (2) inhibiting binding to the specimen support films and (3) reducing compression after drying. Methylcellulose embedment provides effective electron imaging of liposomes, nanodiscs and viruses as well as comprehensive visualization of nanoparticle populations in droplets of known size. These qualities facilitate unbiased sampling, rapid size measurement and estimation of nanoparticle numbers by means of ratio counting using a colloidal gold calibrant. Specimen preparation and quantification take minutes and require a few microliters of sample using only basic laboratory equipment and a standard TEM. | |
dc.format.extent | 13 | |
dc.format.extent | 1461593 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | Electron microscopy | en |
dc.subject | Liposome | en |
dc.subject | Methylcellulose | en |
dc.subject | Nanoparticles | en |
dc.subject | Negative stain | en |
dc.subject | Quantification | en |
dc.subject | Stereology | en |
dc.subject | Virus | en |
dc.subject | QD Chemistry | en |
dc.subject | QH301 Biology | en |
dc.subject | RC Internal medicine | en |
dc.subject | NDAS | en |
dc.subject | BDC | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | RC | en |
dc.title | Nanoparticle suspensions enclosed in methylcellulose : a new approach for quantifying nanoparticles in transmission electron microscopy | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. University of St Andrews | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1038/srep25275 | |
dc.description.status | Peer reviewed | en |
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