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dc.contributor.authorMotion, Claire Louise
dc.contributor.authorLovett, Janet Eleanor
dc.contributor.authorBell, Stacey
dc.contributor.authorCassidy, Scott Lindsay
dc.contributor.authorCruickshank, Paul Alexander Sawchuk
dc.contributor.authorBolton, David Robert
dc.contributor.authorHunter, Robert Iain
dc.contributor.authorEl Mkami, Hassane
dc.contributor.authorVan Doorslaer, Sabine
dc.contributor.authorSmith, Graham Murray
dc.identifier.citationMotion , C L , Lovett , J E , Bell , S , Cassidy , S L , Cruickshank , P A S , Bolton , D R , Hunter , R I , El Mkami , H , Van Doorslaer , S & Smith , G M 2016 , ' DEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPR ' , Journal of Physical Chemistry Letters , vol. 7 , no. 8 , pp. 1411-1415 .
dc.identifier.otherPURE: 241652026
dc.identifier.otherPURE UUID: 9edcefd2-4287-4822-a219-d87594cc1154
dc.identifier.otherScopus: 84967214263
dc.identifier.otherORCID: /0000-0002-3561-450X/work/40301307
dc.identifier.otherORCID: /0000-0001-6578-7412/work/59464750
dc.identifier.otherORCID: /0000-0002-0552-5784/work/60195390
dc.identifier.otherORCID: /0000-0002-0415-9540/work/60195455
dc.identifier.otherWOS: 000374810800001
dc.descriptionClaire Motion would like to acknowledge funding from EPSRC as part of the iMR-CDT. Stacey Bell and Janet Lovett thank EPSRC Grant Number EP/LO22044/1. Janet Lovett also thanks the Royal Society for a University Research Fellowship and Research Grant RG120645. Sabine Van Doorslaer acknowledges the Research Foundation – Flanders (FWO) for financial support (grant G.0687.13). The W-band instrument was developed under the UK Research Council’s Basic Technology Program (grant EP/F039034/1). We also thank the Wellcome Trust (grant 099149/Z/12/Z).en
dc.description.abstractThis work demonstrates the feasibility of making sensitive nanometer distancemeasurements between Fe(III) heme centers and nitroxide spin labels in proteins using the double electron-electron resonance (DEER) pulsed EPR technique at 94 GHz. Techniques to measure accurately long distances in many classes of heme proteins using DEER are currently strongly limited by sensitivity. In this paper we demonstrate sensitivity gains of more than 30times compared with previous lower frequency (X-band) DEER measurements on both human neuroglobin and sperm whale myoglobin. This is achieved by: taking advantage of recent instrumental advances; employing wideband excitation techniques based on composite pulses and exploiting more favorable relaxation properties of low-spin Fe(III) in high magnetic fields. This gain in sensitivity potentially allows the DEER technique to be routinely used as a sensitive probe of structure and conformation in the large number of heme and many other metalloproteins.
dc.relation.ispartofJournal of Physical Chemistry Lettersen
dc.rightsCopyright 2016 American Chemical Society. This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.en
dc.subjectQC Physicsen
dc.subjectQD Chemistryen
dc.titleDEER sensitivity between iron centers and nitroxides in heme-containing proteins improves dramatically using broadband, high-field EPRen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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