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dc.contributor.authorBarr, David A.
dc.contributor.authorKamdolozi, Mercy
dc.contributor.authorNishihara, Yo
dc.contributor.authorNdhlovu, Victor
dc.contributor.authorKhonga, Margaret
dc.contributor.authorDavies, Geraint R.
dc.contributor.authorSloan, Derek James
dc.identifier.citationBarr , D A , Kamdolozi , M , Nishihara , Y , Ndhlovu , V , Khonga , M , Davies , G R & Sloan , D J 2016 , ' Serial image analysis of Mycobacterium tuberculosis colony growth reveals a persistent subpopulation in sputum during treatment of pulmonary TB ' , Tuberculosis , vol. 98 , pp. 110-115 .
dc.identifier.otherPURE: 242076646
dc.identifier.otherPURE UUID: 6d21cb9d-c790-4818-ba7e-a4c297d50103
dc.identifier.otherRIS: urn:A4B1D97B51229F7AC76FD3AA29E891E4
dc.identifier.otherScopus: 84962767546
dc.descriptionThis study received no direct funding. D.A.B. is supported by a Wellcome Trust Clinical PhD studentship (105165/Z/14/A). Tuberculosis Laboratory, College of Medicine, Malawi is supported by PanACEA Biomarker Extension Programe (PANBIOME) (SP.2011.41304.008).en
dc.description.abstractFaster elimination of drug tolerant ‘persister’ bacteria may shorten treatment of tuberculosis (TB) but no method exists to quantify persisters in clinical samples. We used automated image analysis to assess whether studying growth characteristics of individual Mycobacterium tuberculosis colonies from sputum on solid media during early TB treatment facilitates ‘persister’ phenotyping. As Time to Detection (TTD) in liquid culture inversely correlates with total bacterial load we also evaluated the relationship between individual colony growth parameters and TTD. Sputum from TB patients in Malawi was prepared for solid and liquid culture after 0, 2 and 4 weeks of treatment. Serial photography of agar plates was used to measure time to appearance (lag time) and radial growth rate for each colony. Mixed-effects modelling was used to analyse changing growth characteristics from serial samples. 20 patients had colony measurements recorded at ≥1 time-point. Overall lag time increased by 6.5 days between baseline and two weeks (p = 0.0001). Total colony count/ml showed typical biphasic elimination, but long lag time colonies (>20days) had slower, monophasic decline. TTD was associated with minimum lag time (time to appearance of first colony1). Slower elimination of long lag time colonies suggests that these may represent a persister subpopulation of bacilli.
dc.rights© 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (
dc.subjectDrug toleranceen
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.titleSerial image analysis of Mycobacterium tuberculosis colony growth reveals a persistent subpopulation in sputum during treatment of pulmonary TBen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.description.statusPeer revieweden

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