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dc.contributor.authorBrown, Rebecca J.
dc.contributor.authorHolden, Matthew
dc.contributor.authorSpiller, O. Brad
dc.contributor.authorChalker, Victoria J.
dc.date.accessioned2016-03-31T23:01:38Z
dc.date.available2016-03-31T23:01:38Z
dc.date.issued2015-10
dc.identifier.citationBrown , R J , Holden , M , Spiller , O B & Chalker , V J 2015 , ' Development of a multilocus sequence typing scheme for the molecular typing of Mycoplasma pneumoniae ' Journal of Clinical Microbiology , vol. 53 , no. 10 , pp. 3195-3203 . https://doi.org/10.1128/JCM.01301-15en
dc.identifier.issn0095-1137
dc.identifier.otherPURE: 205768209
dc.identifier.otherPURE UUID: 9b6487d1-1d48-4229-bd59-309d82553439
dc.identifier.otherPubMed: 26202118
dc.identifier.otherScopus: 84941985549
dc.identifier.urihttp://hdl.handle.net/10023/8530
dc.descriptionThis work was funded by Public Health England. These studies were supported by funding initiatives by the National Institute for Social Care and Health Research (NISCHR; research support from the Welsh Government) via the registered research group Microbial and Infection Translational Research Group (MITReG) and Children and Young Persons Research Network (CYPRN).en
dc.description.abstractMycoplasma pneumoniae is a major human respiratory pathogen causing both upper and lower respiratory disease in humans of all ages, and it can also result in other serious extrapulmonary sequelae. A multilocus sequence typing (MLST) scheme for M. pneumoniae was developed based on the sequences of eight housekeeping genes (ppa, pgm, gyrB, gmk, glyA, atpA, arcC, and adk) and applied to 55 M. pneumoniae clinical isolates and the two type strains M129 and FH. A total of 12 sequence types (STs) resulted for 57 M. pneumoniae isolates tested, with a discriminatory index of 0.21 STs per isolate. The MLST loci used in this scheme were shown to be stable in 10 strains following 10 sequential subculture passages. Phylogenetic analysis of concatenated sequences of the eight loci indicated two distinct genetic clusters that were directly linked to multilocus variable-number tandem repeat analysis (MLVA) type. Genetic MLST clustering was confirmed by genomic sequence analysis, indicating that the MLST scheme developed in this study is representative of the genome. Furthermore, this MLST scheme was shown to be more discriminatory than both MLVA and P1 typing for the M. pneumoniae isolates examined, providing a method for further and more detailed analysis of observed epidemic peaks of M. pneumoniae infection. This scheme is supported by a public Web-based database (http://pubmlst.org/mpneumoniae).
dc.language.isoeng
dc.relation.ispartofJournal of Clinical Microbiologyen
dc.rightsCopyright © 2015, American Society for Microbiology. All Rights Reserved. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at: https://dx.doi.org/10.1128/JCM.01301-15en
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subjectQH301 Biologyen
dc.subjectQR Microbiologyen
dc.subjectNDASen
dc.subject.lccRA0421en
dc.subject.lccQH301en
dc.subject.lccQRen
dc.titleDevelopment of a multilocus sequence typing scheme for the molecular typing of Mycoplasma pneumoniaeen
dc.typeJournal articleen
dc.description.versionPostprinten
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1128/JCM.01301-15
dc.description.statusPeer revieweden
dc.date.embargoedUntil2016-04-01
dc.identifier.urlhttp://dx.doi.org/10.1128/JCM.01301-15


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