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Dissecting daily and circadian expression rhythms of clock-controlled genes in human blood

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Ackermann_Clock_controlledGenes_JoBR_AM.pdf (884.8Kb)
Date
01/02/2016
Author
Lech, Karolina
Ackermann, Katrin
Revell, Victoria L.
Lao, Oscar
Skene, Debra J.
Kayser, Manfred
Keywords
Sleep deprivation
Constant routine
Gene expression
Human blood
Clock-controlled genes
QD Chemistry
QH426 Genetics
Physiology
Physiology (medical)
NDAS
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Abstract
The identification and investigation of novel clock-controlled genes (CCGs) has been conducted thus far mainly in model organisms such as nocturnal rodents, with limited information in humans. Here, we aimed to characterize daily and circadian expression rhythms of CCGs in human peripheral blood during a sleep/sleep deprivation (S/SD) study and a constant routine (CR) study. Blood expression levels of 9 candidate CCGs (SREBF1, TRIB1, USF1, THRA1, SIRT1, STAT3, CAPRIN1, MKNK2, and ROCK2), were measured across 48 h in 12 participants in the S/SD study and across 33 h in 12 participants in the CR study. Statistically significant rhythms in expression were observed for STAT3, SREBF1, TRIB1, and THRA1 in samples from both the S/SD and the CR studies, indicating that their rhythmicity is driven by the endogenous clock. The MKNK2 gene was significantly rhythmic in the S/SD but not the CR study, which implies its exogenously driven rhythmic expression. In addition, we confirmed the circadian expression of PER1, PER3, and REV-ERBα in the CR study samples, while BMAL1 and HSPA1B were not significantly rhythmic in the CR samples; all 5 genes previously showed significant expression in the S/SD study samples. Overall, our results demonstrate that rhythmic expression patterns of clock and selected clock-controlled genes in human blood cells are in part determined by exogenous factors (sleep and fasting state) and in part by the endogenous circadian timing system. Knowledge of the exogenous and endogenous regulation of gene expression rhythms is needed prior to the selection of potential candidate marker genes for future applications in medical and forensic settings.
Citation
Lech , K , Ackermann , K , Revell , V L , Lao , O , Skene , D J & Kayser , M 2016 , ' Dissecting daily and circadian expression rhythms of clock-controlled genes in human blood ' , Journal of Biological Rhythms , vol. 31 , no. 1 , pp. 68-81 . https://doi.org/10.1177/0748730415611761
Publication
Journal of Biological Rhythms
Status
Peer reviewed
DOI
https://doi.org/10.1177/0748730415611761
ISSN
0748-7304
Type
Journal article
Rights
© 2015 The Author(s). This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://dx.doi.org/10.1177/0748730415611761
Description
This study was supported in part by the Netherlands Organization for Scientific Research (NWO) Forensic Science Program Grant 27.011.001, the European Union 6th Framework project EUCLOCK (018741), the UK Biotechnology and Biological Sciences Research Council (BBSRC) grant BB/I019405/1, and Erasmus MC University Medical Center Rotterdam. D.J.S. is a Royal Society Wolfson Research Merit Award holder.
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  • University of St Andrews Research
URL
http://jbr.sagepub.com/content/31/1/68/suppl/DC1
URI
http://hdl.handle.net/10023/8436

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