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dc.contributor.authorPegoraro, Mirko
dc.contributor.authorBafna, Akanksha
dc.contributor.authorDavies, Nathaniel J.
dc.contributor.authorShuker, David M.
dc.contributor.authorTauber, Eran
dc.identifier.citationPegoraro , M , Bafna , A , Davies , N J , Shuker , D M & Tauber , E 2016 , ' DNA methylation changes induced by long and short photoperiods in Nasonia ' , Genome Research , vol. 26 , no. 2 , pp. 203-210 .
dc.identifier.otherPURE: 241082377
dc.identifier.otherPURE UUID: 5d51108a-f6dc-4998-8201-c1f9c7db9e11
dc.identifier.otherScopus: 84956964023
dc.identifier.otherWOS: 000369341900006
dc.descriptionThis study was partly supported by a Biotechnology and Biological Sciences Research Council (BBSRC) award to E.T. (BB/K001922/1), N.J.D. (BB/J014532/1), and the University of Leicester Open Scholarship to A.B., and also by the Natural Environment Research Council (NE/J024481/1).en
dc.description.abstractMany organisms monitor the annual change in day length and use this information for the timing of their seasonal response. However, the molecular mechanisms underlying photoperiodic timing are largely unknown. The wasp Nasonia vitripennis is an emerging model organism that exhibits a strong photoperiodic response: Short autumnal days experienced by females lead to the induction of developmental arrest (diapause) in their progeny, allowing winter survival of the larvae. How female Nasonia control the developmental trajectory of their offspring is unclear. Here, we took advantage of the recent discovery that DNA methylation is pervasive in Nasonia and tested its role in photoperiodism. We used reduced representation bisulfite sequencing (RRBS) to profile DNA methylation in adult female wasps subjected to different photoperiods and identified substantial differential methylation at the single base level. We also show that knocking down DNA methyltransferase 1a (Dnmt1a), Dnmt3, or blocking DNA methylation pharmacologically, largely disrupts the photoperiodic diapause response of the wasps. To our knowledge, this is the first example for a role of DNA methylation in insect photoperiodic timing.
dc.relation.ispartofGenome Researchen
dc.rights© 2016 Pegoraro et al. This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International).en
dc.subjectQH426 Geneticsen
dc.titleDNA methylation changes induced by long and short photoperiods in Nasoniaen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Centre for Biological Diversityen
dc.description.statusPeer revieweden

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