Files in this item
Localizing the lipid products of PI3Kγ in neutrophils
Item metadata
dc.contributor.author | Norton, Laura | |
dc.contributor.author | Lindsay, Yvonne | |
dc.contributor.author | Deladeriere, Arnaud | |
dc.contributor.author | Chessa, Tamara | |
dc.contributor.author | Guillou, Hervé | |
dc.contributor.author | Suire, Sabine | |
dc.contributor.author | Lucocq, John | |
dc.contributor.author | Walker, Simon | |
dc.contributor.author | Andrews, Simon | |
dc.contributor.author | Segonds-Pichon, Anne | |
dc.contributor.author | Rausch, Oliver | |
dc.contributor.author | Finan, Peter | |
dc.contributor.author | Sasaki, Takehiko | |
dc.contributor.author | Du, Cheng-Jin | |
dc.contributor.author | Bretschneider, Till | |
dc.contributor.author | Ferguson, G John | |
dc.contributor.author | Hawkins, Phillip T | |
dc.contributor.author | Stephens, Len | |
dc.date.accessioned | 2016-02-17T12:40:07Z | |
dc.date.available | 2016-02-17T12:40:07Z | |
dc.date.issued | 2016-01 | |
dc.identifier | 237837416 | |
dc.identifier | 53ed1298-8769-452a-8dc2-17ae95988e20 | |
dc.identifier | 26596865 | |
dc.identifier | 84958745971 | |
dc.identifier.citation | Norton , L , Lindsay , Y , Deladeriere , A , Chessa , T , Guillou , H , Suire , S , Lucocq , J , Walker , S , Andrews , S , Segonds-Pichon , A , Rausch , O , Finan , P , Sasaki , T , Du , C-J , Bretschneider , T , Ferguson , G J , Hawkins , P T & Stephens , L 2016 , ' Localizing the lipid products of PI3Kγ in neutrophils ' , Advances in Biological Regulation , vol. 60 , pp. 36-45 . https://doi.org/10.1016/j.jbior.2015.10.005 | en |
dc.identifier.issn | 2212-4934 | |
dc.identifier.other | ORCID: /0000-0002-5191-0093/work/64361206 | |
dc.identifier.uri | https://hdl.handle.net/10023/8248 | |
dc.description.abstract | Class I phosphoinositide 3-kinases (PI3Ks) are important regulators of neutrophil migration in response to a range of chemoattractants. Their primary lipid products PtdIns(3,4,5)P3 and PtdIns(3,4)P2 preferentially accumulate near to the leading edge of migrating cells and are thought to act as an important cue organizing molecular and morphological polarization. We have investigated the distribution and accumulation of these lipids independently in mouse neutrophils using eGFP-PH reporters and electron microscopy (EM). We found that authentic mouse neutrophils rapidly polarized their Class I PI3K signalling, as read-out by eGFP-PH reporters, both at the up-gradient leading edge in response to local stimulation with fMLP as well as spontaneously and randomly in response to uniform stimulation. EM studies revealed these events occurred at the plasma membrane, were dominated by accumulation of PtdIns(3,4,5)P3, but not PtdIns(3,4)P2, and were dependent on PI3Kγ and its upstream activation by both Ras and Gβγs. | |
dc.format.extent | 1915115 | |
dc.language.iso | eng | |
dc.relation.ispartof | Advances in Biological Regulation | en |
dc.subject | PI3K | en |
dc.subject | Neutrophil | en |
dc.subject | Polarization | en |
dc.subject | R Medicine | en |
dc.subject | QH301 Biology | en |
dc.subject | NDAS | en |
dc.subject.lcc | R | en |
dc.subject.lcc | QH301 | en |
dc.title | Localizing the lipid products of PI3Kγ in neutrophils | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1016/j.jbior.2015.10.005 | |
dc.description.status | Peer reviewed | en |
This item appears in the following Collection(s)
Items in the St Andrews Research Repository are protected by copyright, with all rights reserved, unless otherwise indicated.