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dc.contributor.authorNorton, Laura
dc.contributor.authorLindsay, Yvonne
dc.contributor.authorDeladeriere, Arnaud
dc.contributor.authorChessa, Tamara
dc.contributor.authorGuillou, Hervé
dc.contributor.authorSuire, Sabine
dc.contributor.authorLucocq, John
dc.contributor.authorWalker, Simon
dc.contributor.authorAndrews, Simon
dc.contributor.authorSegonds-Pichon, Anne
dc.contributor.authorRausch, Oliver
dc.contributor.authorFinan, Peter
dc.contributor.authorSasaki, Takehiko
dc.contributor.authorDu, Cheng-Jin
dc.contributor.authorBretschneider, Till
dc.contributor.authorFerguson, G John
dc.contributor.authorHawkins, Phillip T
dc.contributor.authorStephens, Len
dc.date.accessioned2016-02-17T12:40:07Z
dc.date.available2016-02-17T12:40:07Z
dc.date.issued2016-01
dc.identifier.citationNorton , L , Lindsay , Y , Deladeriere , A , Chessa , T , Guillou , H , Suire , S , Lucocq , J , Walker , S , Andrews , S , Segonds-Pichon , A , Rausch , O , Finan , P , Sasaki , T , Du , C-J , Bretschneider , T , Ferguson , G J , Hawkins , P T & Stephens , L 2016 , ' Localizing the lipid products of PI3Kγ in neutrophils ' , Advances in Biological Regulation , vol. 60 , pp. 36-45 . https://doi.org/10.1016/j.jbior.2015.10.005en
dc.identifier.issn2212-4934
dc.identifier.otherPURE: 237837416
dc.identifier.otherPURE UUID: 53ed1298-8769-452a-8dc2-17ae95988e20
dc.identifier.otherPubMed: 26596865
dc.identifier.otherScopus: 84958745971
dc.identifier.otherORCID: /0000-0002-5191-0093/work/64361206
dc.identifier.urihttps://hdl.handle.net/10023/8248
dc.description.abstractClass I phosphoinositide 3-kinases (PI3Ks) are important regulators of neutrophil migration in response to a range of chemoattractants. Their primary lipid products PtdIns(3,4,5)P3 and PtdIns(3,4)P2 preferentially accumulate near to the leading edge of migrating cells and are thought to act as an important cue organizing molecular and morphological polarization. We have investigated the distribution and accumulation of these lipids independently in mouse neutrophils using eGFP-PH reporters and electron microscopy (EM). We found that authentic mouse neutrophils rapidly polarized their Class I PI3K signalling, as read-out by eGFP-PH reporters, both at the up-gradient leading edge in response to local stimulation with fMLP as well as spontaneously and randomly in response to uniform stimulation. EM studies revealed these events occurred at the plasma membrane, were dominated by accumulation of PtdIns(3,4,5)P3, but not PtdIns(3,4)P2, and were dependent on PI3Kγ and its upstream activation by both Ras and Gβγs.
dc.language.isoeng
dc.relation.ispartofAdvances in Biological Regulationen
dc.rights© 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/ 4.0/).en
dc.subjectPI3Ken
dc.subjectNeutrophilen
dc.subjectPolarizationen
dc.subjectR Medicineen
dc.subjectQH301 Biologyen
dc.subjectNDASen
dc.subject.lccRen
dc.subject.lccQH301en
dc.titleLocalizing the lipid products of PI3Kγ in neutrophilsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1016/j.jbior.2015.10.005
dc.description.statusPeer revieweden


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