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dc.contributor.authorMinskaia, Ekaterina
dc.contributor.authorLuke, Garry Alec
dc.identifier.citationMinskaia , E & Luke , G A 2015 , ' 2A - the "go-to" technology for transgene co-expression ' , Single Cell Biology , vol. S1 , no. 004 , pp. 1-3 .
dc.identifier.otherPURE: 221892789
dc.identifier.otherPURE UUID: 4deb3847-2e39-4d8a-a243-bae39462efe0
dc.description.abstractIn order to co-express multiple genes for biotechnological and biomedical applications, several approaches have been used with varying degrees of success. Currently, internal ribosome entry site (IRES) elements and “self-cleaving” 2A peptides are the most widely used. The length of the IRES can be prohibitive and IRES-dependent translation of the second open reading frame is often significantly reduced. 2A peptides have gained in popularity due to their small size and ability to consistently produce discrete proteins at an equal level. Here, we promote the use of these sequences as the “go-to” technology for co-expression of multiple proteins.
dc.relation.ispartofSingle Cell Biologyen
dc.rights©2015 Minskaia E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectProtein co-expressionen
dc.subjectQH301 Biologyen
dc.subjectQR Microbiologyen
dc.title2A - the "go-to" technology for transgene co-expressionen
dc.typeJournal itemen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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