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dc.contributor.authorMinskaia, Ekaterina
dc.contributor.authorLuke, Garry Alec
dc.date.accessioned2016-02-03T17:10:05Z
dc.date.available2016-02-03T17:10:05Z
dc.date.issued2015-08-26
dc.identifier221892789
dc.identifier4deb3847-2e39-4d8a-a243-bae39462efe0
dc.identifier.citationMinskaia , E & Luke , G A 2015 , ' 2A - the "go-to" technology for transgene co-expression ' , Single Cell Biology , vol. S1 , no. 004 , pp. 1-3 . https://doi.org/10.4172/2168-9431.S1-004en
dc.identifier.urihttps://hdl.handle.net/10023/8125
dc.description.abstractIn order to co-express multiple genes for biotechnological and biomedical applications, several approaches have been used with varying degrees of success. Currently, internal ribosome entry site (IRES) elements and “self-cleaving” 2A peptides are the most widely used. The length of the IRES can be prohibitive and IRES-dependent translation of the second open reading frame is often significantly reduced. 2A peptides have gained in popularity due to their small size and ability to consistently produce discrete proteins at an equal level. Here, we promote the use of these sequences as the “go-to” technology for co-expression of multiple proteins.
dc.format.extent471586
dc.language.isoeng
dc.relation.ispartofSingle Cell Biologyen
dc.subjectProtein co-expressionen
dc.subject2Aen
dc.subjectBiotechnologyen
dc.subjectBiomedicineen
dc.subjectQH301 Biologyen
dc.subjectQR Microbiologyen
dc.subject.lccQH301en
dc.subject.lccQRen
dc.title2A - the "go-to" technology for transgene co-expressionen
dc.typeJournal itemen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.4172/2168-9431.S1-004
dc.description.statusPeer revieweden


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