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Asymmetric distribution of pl10 and bruno2, new members of a conserved core of early germline determinants in cephalochordates
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dc.contributor.author | Dailey, Simon Christopher | |
dc.contributor.author | Planas, Roser Febrero | |
dc.contributor.author | Espier, Adriana Rossell | |
dc.contributor.author | Garcia-Fernandez, Jordi | |
dc.contributor.author | Somorjai, Ildiko Maureen Lara | |
dc.date.accessioned | 2016-01-22T14:10:15Z | |
dc.date.available | 2016-01-22T14:10:15Z | |
dc.date.issued | 2016-01-22 | |
dc.identifier | 240101370 | |
dc.identifier | 3687712c-750f-4ae0-a552-14c7ba5234d6 | |
dc.identifier | 85021098701 | |
dc.identifier | 000452060500010 | |
dc.identifier.citation | Dailey , S C , Planas , R F , Espier , A R , Garcia-Fernandez , J & Somorjai , I M L 2016 , ' Asymmetric distribution of pl10 and bruno2 , new members of a conserved core of early germline determinants in cephalochordates ' , Frontiers in Ecology and Evolution , vol. 3 , 156 . https://doi.org/10.3389/fevo.2015.00156 | en |
dc.identifier.issn | 2296-701X | |
dc.identifier.other | ORCID: /0000-0001-5243-6664/work/46168671 | |
dc.identifier.uri | https://hdl.handle.net/10023/8062 | |
dc.description | SD is funded through a MASTS PhD studentship (St Andrews, UK). IMLS gratefully acknowledges previous funding from Marie Curie IEF postdoctoral fellowship, FP7 People Programme (Barcelona, Spain); as well as MASTS (Marine Alliance for Science and Technology Scotland) laboratory start-up funds (St Andrews, UK). | en |
dc.description.abstract | Molecular fingerprinting of conserved germline and somatic ¨stemness¨ markers in different taxa have been key in defining the mechanism of germline specification ("preformation" or "epigenesis"), as well as expression domains of somatic progenitors. The distribution of molecular markers for primordial germ cells (PGCs), including vasa, nanos and piwil1, as well as Vasa antibody staining, support a determinative mechanism of germline specification in the cephalochordate Branchiostoma lanceolatum, similarly to other amphioxus species. pl10 and bruno2, but not bruno4/6, are also expressed in a pattern consistent with these other germline genes, adding to our repertoire of PGC markers in lancelets. Expression of nanos, vasa and the remaining markers (musashi, pufA, pufB, pumilio and piwil2) may define populations of putative somatic progenitors in the tailbud, the amphioxus posterior growth zone, or zones of proliferative activity. Finally, we also identify a novel expression domain for musashi, a classic neural stem cell marker, during notochord development in amphioxus. These results are discussed in the context of germline determination in other taxa, stem cell regulation and regenerative capacity in adult amphioxus. | |
dc.format.extent | 5437088 | |
dc.language.iso | eng | |
dc.relation.ispartof | Frontiers in Ecology and Evolution | en |
dc.subject | Amphioxus | en |
dc.subject | Evolution | en |
dc.subject | Regeneration | en |
dc.subject | PGCs | en |
dc.subject | Posterior stem cells | en |
dc.subject | Preformation | en |
dc.subject | QH301 Biology | en |
dc.subject.lcc | QH301 | en |
dc.title | Asymmetric distribution of pl10 and bruno2, new members of a conserved core of early germline determinants in cephalochordates | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Marine Alliance for Science & Technology Scotland | en |
dc.contributor.institution | University of St Andrews. Scottish Oceans Institute | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.3389/fevo.2015.00156 | |
dc.description.status | Peer reviewed | en |
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