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dc.contributor.authorZhang, Jing
dc.contributor.authorGraham, Shirley
dc.contributor.authorTello, Agnes
dc.contributor.authorLiu, Huanting
dc.contributor.authorWhite, Malcolm Frederick
dc.date.accessioned2016-01-22T12:10:05Z
dc.date.available2016-01-22T12:10:05Z
dc.date.issued2016-02-29
dc.identifier.citationZhang , J , Graham , S , Tello , A , Liu , H & White , M F 2016 , ' Multiple nucleic acid cleavage modes in divergent type III CRISPR systems ' , Nucleic Acids Research , vol. 44 , no. 4 , pp. 1789-1799 . https://doi.org/10.1093/nar/gkw020en
dc.identifier.issn0305-1048
dc.identifier.otherPURE: 240255612
dc.identifier.otherPURE UUID: 481c75bf-3f73-4a50-badb-89c660202734
dc.identifier.otherScopus: 84960540026
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136085
dc.identifier.otherWOS: 000371519700035
dc.identifier.urihttps://hdl.handle.net/10023/8058
dc.description.abstractCRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of transcription-dependent DNA targeting. The crenarchaeon Sulfolobus solfataricus has two divergent subtypes of the type III system (Sso-IIID and a Cmr7-containing variant of Sso-IIIB). Here, we report that both the Sso-IIID and Sso-IIIB complexes cleave cognate RNA targets with a ruler mechanism and 6 or 12 nt spacing that relates to the organization of the Cas7 backbone. This backbone-mediated cleavage activity thus appears universal for the type III systems. The Sso-IIIB complex is also known to possess a distinct ‘UA’ cleavage mode. The predominant activity observed in vitro depends on the relative molar concentration of protein and target RNA. The Sso-IIID complex can cleave plasmid DNA targets in vitro, generating linear DNA products with an activity that is dependent on both the cyclase and HD nuclease domains of the Cas10 subunit, suggesting a role for both nuclease active sites in the degradation of double-stranded DNA targets.
dc.format.extent11
dc.language.isoeng
dc.relation.ispartofNucleic Acids Researchen
dc.rightsCopyright The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comen
dc.subjectQH301 Biologyen
dc.subjectQD Chemistryen
dc.subject.lccQH301en
dc.subject.lccQDen
dc.titleMultiple nucleic acid cleavage modes in divergent type III CRISPR systemsen
dc.typeJournal articleen
dc.contributor.sponsorBBSRCen
dc.contributor.sponsorBBSRCen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.identifier.doihttps://doi.org/10.1093/nar/gkw020
dc.description.statusPeer revieweden
dc.identifier.grantnumberBB/K000314/1en
dc.identifier.grantnumberBB/M000400/1en


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