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Genome-wide DNA methylation patterns in wild samples of two morphotypes of threespine stickleback (Gasterosteus aculeatus)
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dc.contributor.author | Smith, Gilbert | |
dc.contributor.author | Smith, Carl | |
dc.contributor.author | Kenny, John | |
dc.contributor.author | Chaudhuri, Roy | |
dc.contributor.author | Ritchie, Michael Gordon | |
dc.date.accessioned | 2015-12-22T14:40:03Z | |
dc.date.available | 2015-12-22T14:40:03Z | |
dc.date.issued | 2015-04 | |
dc.identifier | 160649930 | |
dc.identifier | 3374ce3f-e3af-4a56-a64f-daaac088e6fb | |
dc.identifier | 84926680972 | |
dc.identifier | 000353560900005 | |
dc.identifier.citation | Smith , G , Smith , C , Kenny , J , Chaudhuri , R & Ritchie , M G 2015 , ' Genome-wide DNA methylation patterns in wild samples of two morphotypes of threespine stickleback ( Gasterosteus aculeatus ) ' , Molecular Biology and Evolution , vol. 32 , no. 4 , pp. 888-895 . https://doi.org/10.1093/molbev/msu344 | en |
dc.identifier.issn | 0737-4038 | |
dc.identifier.other | ORCID: /0000-0003-3285-0379/work/47136223 | |
dc.identifier.other | ORCID: /0000-0001-7913-8675/work/46761165 | |
dc.identifier.uri | https://hdl.handle.net/10023/7919 | |
dc.description | This work was supported by the Natural Environment Research Council (NERC) through NERC Biomolecular Analysis Facility (NBAF) grant NBAF612. | en |
dc.description.abstract | Epigenetic marks such as DNA methylation play important biological roles in gene expression regulation and cellular differentiation during development. To examine whether DNA methylation patterns are potentially associated with naturally occurring phenotypic differences, we examined genome-wide DNA methylation within G. aculeatus, using reduced representation bisulfite sequencing (RRBS). First, we identified highly methylated regions of the stickleback genome, finding such regions to be located predominantly within genes, and associated with genes functioning in metabolism and biosynthetic processes, cell adhesion, signaling pathways and blood vessel development. Next, we identified putative differentially methylated regions (DMRs) of the genome between complete and low lateral plate morphs of G. aculeatus. We detected 77 DMRs that were mainly located in intergenic regions. Annotations of genes associated with these DMRs revealed potential functions in a number of known divergent adaptive phenotypes between G. aculeatus ecotypes, including cardiovascular development, growth and neuromuscular development. | |
dc.format.extent | 191734 | |
dc.language.iso | eng | |
dc.relation.ispartof | Molecular Biology and Evolution | en |
dc.subject | Adaptation | en |
dc.subject | Lateral plate morph | en |
dc.subject | Ecotype | en |
dc.subject | Epigenetic mechanism | en |
dc.subject | Phenotypic plasticity | en |
dc.subject | QH301 Biology | en |
dc.subject | QH426 Genetics | en |
dc.subject | DAS | en |
dc.subject.lcc | QH301 | en |
dc.subject.lcc | QH426 | en |
dc.title | Genome-wide DNA methylation patterns in wild samples of two morphotypes of threespine stickleback (Gasterosteus aculeatus) | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Marine Alliance for Science & Technology Scotland | en |
dc.contributor.institution | University of St Andrews. Scottish Oceans Institute | en |
dc.contributor.institution | University of St Andrews. Centre for Biological Diversity | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.identifier.doi | 10.1093/molbev/msu344 | |
dc.description.status | Peer reviewed | en |
dc.date.embargoedUntil | 2015-12-21 |
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