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dc.contributor.authorEves-van den Akker, Sebastian
dc.contributor.authorLilley, Catherine J.
dc.contributor.authorJones, John T.
dc.contributor.authorUrwin, Peter E.
dc.identifier.citationEves-van den Akker , S , Lilley , C J , Jones , J T & Urwin , P E 2014 , ' Identification and characterisation of a hyper-variable apoplastic effector gene family of the potato cyst nematodes ' , PLoS Pathogens , vol. 10 , no. 9 , e1004391 .
dc.descriptionDate of Acceptance: 11/08/2014en
dc.description.abstractSedentary endoparasitic nematodes are obligate biotrophs that modify host root tissues, using a suite of effector proteins to create and maintain a feeding site that is their sole source of nutrition. Using assumptions about the characteristics of genes involved in plant-nematode biotrophic interactions to inform the identification strategy, we provide a description and characterisation of a novel group of hyper-variable extracellular effectors termed HYP, from the potato cyst nematode Globodera pallida. HYP effectors comprise a large gene family, with a modular structure, and have unparalleled diversity between individuals of the same population: no two nematodes tested had the same genetic complement of HYP effectors. Individuals vary in the number, size, and type of effector subfamilies. HYP effectors are expressed throughout the biotrophic stages in large secretory cells associated with the amphids of parasitic stage nematodes as confirmed by in situ hybridisation. The encoded proteins are secreted into the host roots where they are detectable by immunochemistry in the apoplasm, between the anterior end of the nematode and the feeding site. We have identified HYP effectors in three genera of plant parasitic nematodes capable of infecting a broad range of mono-and dicotyledon crop species. In planta RNAi targeted to all members of the effector family causes a reduction in successful parasitism.
dc.relation.ispartofPLoS Pathogensen
dc.subjectPlant-parasitic nematodesen
dc.subjectCytochrome-p450 genesen
dc.subjectQH301 Biologyen
dc.titleIdentification and characterisation of a hyper-variable apoplastic effector gene family of the potato cyst nematodesen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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