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Whole-central nervous system functional imaging in larval Drosophila
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dc.contributor.author | Lemon, William | |
dc.contributor.author | Pulver, Stefan | |
dc.contributor.author | Hockendorf, Burkhard | |
dc.contributor.author | McDole, Katie | |
dc.contributor.author | Branson, Kristin | |
dc.contributor.author | Freeman, Jeremy | |
dc.contributor.author | Keller, Phillip | |
dc.date.accessioned | 2015-09-21T10:40:02Z | |
dc.date.available | 2015-09-21T10:40:02Z | |
dc.date.issued | 2015-08-11 | |
dc.identifier.citation | Lemon , W , Pulver , S , Hockendorf , B , McDole , K , Branson , K , Freeman , J & Keller , P 2015 , ' Whole-central nervous system functional imaging in larval Drosophila ' , Nature Communications , vol. 6 , 7924 . https://doi.org/10.1038/ncomms8924 | en |
dc.identifier.issn | 2041-1723 | |
dc.identifier.other | PURE: 217971593 | |
dc.identifier.other | PURE UUID: 25844d85-8b47-4c5b-9f7f-4afd6bfd5dbb | |
dc.identifier.other | Scopus: 84939132208 | |
dc.identifier.other | ORCID: /0000-0001-5170-7522/work/69463424 | |
dc.identifier.uri | https://hdl.handle.net/10023/7516 | |
dc.description | This work was supported by the Howard Hughes Medical Institute. | en |
dc.description.abstract | Understanding how the brain works in tight concert with the rest of the central nervous system (CNS) hinges upon knowledge of coordinated activity patterns across the whole CNS. We present a method for measuring activity in an entire, non-transparent CNS with high spatiotemporal resolution. We combine a light-sheet microscope capable of simultaneous multi-view imaging at volumetric speeds 25-fold faster than the state-of-the-art, a whole-CNS imaging assay for the isolated Drosophila larval CNS and a computational framework for analysing multi-view, whole-CNS calcium imaging data. We image both brain and ventral nerve cord, covering the entire CNS at 2 or 5 Hz with two- or one-photon excitation, respectively. By mapping network activity during fictive behaviours and quantitatively comparing high-resolution whole-CNS activity maps across individuals, we predict functional connections between CNS regions and reveal neurons in the brain that identify type and temporal state of motor programs executed in the ventral nerve cord. | |
dc.format.extent | 16 | |
dc.language.iso | eng | |
dc.relation.ispartof | Nature Communications | en |
dc.rights | Copyright © 2015 the Authors. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | en |
dc.subject | RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry | en |
dc.subject | DAS | en |
dc.subject | BDC | en |
dc.subject | R2C | en |
dc.subject.lcc | RC0321 | en |
dc.title | Whole-central nervous system functional imaging in larval Drosophila | en |
dc.type | Journal article | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Psychology and Neuroscience | en |
dc.identifier.doi | https://doi.org/10.1038/ncomms8924 | |
dc.description.status | Peer reviewed | en |
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