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dc.contributor.authorPuxty, Richard J.
dc.contributor.authorPerez-Sepulveda, Blanca
dc.contributor.authorRihtman, Branko
dc.contributor.authorEvans, David J.
dc.contributor.authorMillard, Andrew D.
dc.contributor.authorScanlan, David J.
dc.date.accessioned2015-09-16T16:10:07Z
dc.date.available2015-09-16T16:10:07Z
dc.date.issued2015-07-15
dc.identifier211241410
dc.identifier50121356-6409-4008-8b01-b334cc0b5e95
dc.identifier000358197600136
dc.identifier84941362509
dc.identifier.citationPuxty , R J , Perez-Sepulveda , B , Rihtman , B , Evans , D J , Millard , A D & Scanlan , D J 2015 , ' Spontaneous deletion of an "ORFanage" region facilitates host adaptation in a "photosynthetic" cyanophage ' , PLoS One , vol. 10 , no. 7 , e0132642 . https://doi.org/10.1371/journal.pone.0132642en
dc.identifier.issn1932-6203
dc.identifier.otherORCID: /0000-0002-1315-4258/work/104252507
dc.identifier.urihttps://hdl.handle.net/10023/7494
dc.description.abstractViruses have been suggested to be the largest source of genetic diversity on Earth. Genome sequencing and metagenomic surveys reveal that novel genes with unknown functions are abundant in viral genomes. Yet few observations exist for the processes and frequency by which these genes are gained and lost. The surface waters of marine environments are dominated by marine picocyanobacteria and their co-existing viruses (cyanophages). Recent genome sequencing of cyanophages has revealed a vast array of genes that have been acquired from their cyanobacterial hosts. Here, we re-sequenced the cyanophage S-PM2 genome after 10 years of near continuous passage through its marine Synechococcus host. During this time a spontaneous mutant (S-PM2d) lacking 13% of the S-PM2 ORFs became dominant in the cyanophage population. These ORFs are found at one loci and are not homologous to any proteins in any other sequenced organism (ORFans). We demonstrate a fitness cost to S-PM2(WT) associated with possession of these ORFs under standard laboratory growth. Metagenomic surveys reveal these ORFs are present in various aquatic environments, are likely of cyanophage origin and appear to be enriched in environments from the extremes of salinity (freshwater and hypersaline). We posit that these ORFs contribute to the flexible gene content of cyanophages and offer a distinct fitness advantage in freshwater and hypersaline environments.
dc.format.extent13
dc.format.extent7013097
dc.language.isoeng
dc.relation.ispartofPLoS Oneen
dc.subjectMarine virusesen
dc.subjectAntagonistic coevolutionen
dc.subjectT4-like myovirusesen
dc.subjectSynechococcusen
dc.subjectGenomeen
dc.subjectQH301 Biologyen
dc.subjectSDG 14 - Life Below Wateren
dc.subject.lccQH301en
dc.titleSpontaneous deletion of an "ORFanage" region facilitates host adaptation in a "photosynthetic" cyanophageen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1371/journal.pone.0132642
dc.description.statusPeer revieweden


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