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Spontaneous deletion of an "ORFanage" region facilitates host adaptation in a "photosynthetic" cyanophage
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dc.contributor.author | Puxty, Richard J. | |
dc.contributor.author | Perez-Sepulveda, Blanca | |
dc.contributor.author | Rihtman, Branko | |
dc.contributor.author | Evans, David J. | |
dc.contributor.author | Millard, Andrew D. | |
dc.contributor.author | Scanlan, David J. | |
dc.date.accessioned | 2015-09-16T16:10:07Z | |
dc.date.available | 2015-09-16T16:10:07Z | |
dc.date.issued | 2015-07-15 | |
dc.identifier | 211241410 | |
dc.identifier | 50121356-6409-4008-8b01-b334cc0b5e95 | |
dc.identifier | 000358197600136 | |
dc.identifier | 84941362509 | |
dc.identifier.citation | Puxty , R J , Perez-Sepulveda , B , Rihtman , B , Evans , D J , Millard , A D & Scanlan , D J 2015 , ' Spontaneous deletion of an "ORFanage" region facilitates host adaptation in a "photosynthetic" cyanophage ' , PLoS One , vol. 10 , no. 7 , e0132642 . https://doi.org/10.1371/journal.pone.0132642 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | ORCID: /0000-0002-1315-4258/work/104252507 | |
dc.identifier.uri | https://hdl.handle.net/10023/7494 | |
dc.description.abstract | Viruses have been suggested to be the largest source of genetic diversity on Earth. Genome sequencing and metagenomic surveys reveal that novel genes with unknown functions are abundant in viral genomes. Yet few observations exist for the processes and frequency by which these genes are gained and lost. The surface waters of marine environments are dominated by marine picocyanobacteria and their co-existing viruses (cyanophages). Recent genome sequencing of cyanophages has revealed a vast array of genes that have been acquired from their cyanobacterial hosts. Here, we re-sequenced the cyanophage S-PM2 genome after 10 years of near continuous passage through its marine Synechococcus host. During this time a spontaneous mutant (S-PM2d) lacking 13% of the S-PM2 ORFs became dominant in the cyanophage population. These ORFs are found at one loci and are not homologous to any proteins in any other sequenced organism (ORFans). We demonstrate a fitness cost to S-PM2(WT) associated with possession of these ORFs under standard laboratory growth. Metagenomic surveys reveal these ORFs are present in various aquatic environments, are likely of cyanophage origin and appear to be enriched in environments from the extremes of salinity (freshwater and hypersaline). We posit that these ORFs contribute to the flexible gene content of cyanophages and offer a distinct fitness advantage in freshwater and hypersaline environments. | |
dc.format.extent | 13 | |
dc.format.extent | 7013097 | |
dc.language.iso | eng | |
dc.relation.ispartof | PLoS One | en |
dc.subject | Marine viruses | en |
dc.subject | Antagonistic coevolution | en |
dc.subject | T4-like myoviruses | en |
dc.subject | Synechococcus | en |
dc.subject | Genome | en |
dc.subject | QH301 Biology | en |
dc.subject | SDG 14 - Life Below Water | en |
dc.subject.lcc | QH301 | en |
dc.title | Spontaneous deletion of an "ORFanage" region facilitates host adaptation in a "photosynthetic" cyanophage | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1371/journal.pone.0132642 | |
dc.description.status | Peer reviewed | en |
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