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dc.contributor.authorLowry, Kym
dc.contributor.authorWoodman, Andrew
dc.contributor.authorCook, Jonathan
dc.contributor.authorEvans, David J.
dc.date.accessioned2015-09-16T15:40:02Z
dc.date.available2015-09-16T15:40:02Z
dc.date.issued2014-06-12
dc.identifier.citationLowry , K , Woodman , A , Cook , J & Evans , D J 2014 , ' Recombination in enteroviruses is a biphasic replicative process involving the generation of greater-than genome length 'imprecise' intermediates ' PLoS Pathogens , vol. 10 , no. 6 , e1004191 . https://doi.org/10.1371/journal.ppat.1004191en
dc.identifier.issn1553-7366
dc.identifier.otherPURE: 211241441
dc.identifier.otherPURE UUID: 4366983f-3f87-4994-aaf8-71a02f96f39b
dc.identifier.otherWOS: 000338197400026
dc.identifier.otherScopus: 84903480766
dc.identifier.urihttp://hdl.handle.net/10023/7490
dc.description.abstractRecombination in enteroviruses provides an evolutionary mechanism for acquiring extensive regions of novel sequence, is suggested to have a role in genotype diversity and is known to have been key to the emergence of novel neuropathogenic variants of poliovirus. Despite the importance of this evolutionary mechanism, the recombination process remains relatively poorly understood. We investigated heterologous recombination using a novel reverse genetic approach that resulted in the isolation of intermediate chimeric intertypic polioviruses bearing genomes with extensive duplicated sequences at the recombination junction. Serial passage of viruses exhibiting such imprecise junctions yielded progeny with increased fitness which had lost the duplicated sequences. Mutations or inhibitors that changed polymerase fidelity or the coalescence of replication complexes markedly altered the yield of recombinants (but did not influence non-replicative recombination) indicating both that the process is replicative and that it may be possible to enhance or reduce recombination-mediated viral evolution if required. We propose that extant recombinants result from a biphasic process in which an initial recombination event is followed by a process of resolution, deleting extraneous sequences and optimizing viral fitness. This process has implications for our wider understanding of 'evolution by duplication' in the positive-strand RNA viruses.
dc.format.extent17
dc.language.isoeng
dc.relation.ispartofPLoS Pathogensen
dc.rightsCopyright 2014 Lowry et al. This is an open-access article distributed under the terms of the Creative Commons unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectVaccine-derived poliovirusen
dc.subjectRibosomal entry siteen
dc.subjectFrequent recombinationen
dc.subjectIn-vitroen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleRecombination in enteroviruses is a biphasic replicative process involving the generation of greater-than genome length 'imprecise' intermediatesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1371/journal.ppat.1004191
dc.description.statusPeer revieweden


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