Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorGarcia de la Serrana Castillo, Daniel
dc.contributor.authorDevlin, Robert
dc.contributor.authorJohnston, Ian Alistair
dc.date.accessioned2015-08-12T13:40:02Z
dc.date.available2015-08-12T13:40:02Z
dc.date.issued2015-07-31
dc.identifier.citationGarcia de la Serrana Castillo , D , Devlin , R & Johnston , I A 2015 , ' RNAseq analysis of fast skeletal muscle in restriction-fed transgenic coho salmon (Oncorhynchus kisutch) : an experimental model uncoupling the growth hormone and nutritional signals regulating growth ' , BMC Genomics , vol. 16 , no. 15 , 564 . https://doi.org/10.1186/s12864-015-1782-zen
dc.identifier.issn1471-2164
dc.identifier.otherPURE: 207292055
dc.identifier.otherPURE UUID: 23b3dc9f-3d74-4f94-849a-4f38b3463644
dc.identifier.otherScopus: 84938097255
dc.identifier.otherORCID: /0000-0002-7796-5754/work/47136015
dc.identifier.otherWOS: 000358760400003
dc.identifier.urihttps://hdl.handle.net/10023/7196
dc.description.abstractBackground Coho salmon (Oncorhynchus kisutch) transgenic for growth hormone (Gh) express Gh in multiple tissues which results in increased appetite and continuous high growth with satiation feeding. Restricting Gh-transgenics to the same lower ration (TR) as wild-type fish (WT) results in similar growth, but with the recruitment of fewer, larger diameter, muscle skeletal fibres to reach a given body size. In order to better understand the genetic mechanisms behind these different patterns of muscle growth and to investigate how the decoupling of Gh and nutritional signals affects gene regulation we used RNA-seq to compare the fast skeletal muscle transcriptome in TR and WT coho salmon. Results Illumina sequencing of individually barcoded libraries from 6 WT and 6 TR coho salmon yielded 704,550,985 paired end reads which were used to construct 323,115 contigs containing 19,093 unique genes of which >10,000 contained >90 % of the coding sequence. Transcripts coding for 31 genes required for myoblast fusion were identified with 22 significantly downregulated in TR relative to WT fish, including 10 (vaspa, cdh15, graf1, crk, crkl, dock1, trio, plekho1a, cdc42a and dock5) associated with signaling through the cell surface protein cadherin. Nineteen out of 44 (43 %) translation initiation factors and 14 of 47 (30 %) protein chaperones were upregulated in TR relative to WT fish. Conclusions TR coho salmon showed increased growth hormone transcripts and gene expression associated with protein synthesis and folding than WT fish even though net rates of protein accretion were similar. The uncoupling of Gh and amino acid signals likely results in additional costs of transcription associated with protein turnover in TR fish. The predicted reduction in the ionic costs of homeostasis in TR fish associated with increased fibre size were shown to involve multiple pathways regulating myotube fusion, particularly cadherin signaling.
dc.format.extent8
dc.language.isoeng
dc.relation.ispartofBMC Genomicsen
dc.rights© 2015 de la serrana et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.subjectSalmonen
dc.subjectGrowthen
dc.subjectTeleost fishen
dc.subjectGrowth hormone transgenicsen
dc.subjectSkeletal muscle transcriptomeen
dc.subjectFish nutritionen
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleRNAseq analysis of fast skeletal muscle in restriction-fed transgenic coho salmon (Oncorhynchus kisutch) : an experimental model uncoupling the growth hormone and nutritional signals regulating growthen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews. Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews. Centre for Research into Ecological & Environmental Modellingen
dc.identifier.doihttps://doi.org/10.1186/s12864-015-1782-z
dc.description.statusPeer revieweden


This item appears in the following Collection(s)

Show simple item record