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Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane
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dc.contributor.author | Li, X. | |
dc.contributor.author | Gu, Y. | |
dc.contributor.author | Dong, H. | |
dc.contributor.author | Wang, W. | |
dc.contributor.author | Dong, C. | |
dc.date.accessioned | 2015-07-24T09:40:02Z | |
dc.date.available | 2015-07-24T09:40:02Z | |
dc.date.issued | 2015-07-07 | |
dc.identifier | 204802561 | |
dc.identifier | 105ef858-35c5-4171-be76-2174a9eae608 | |
dc.identifier | 84936797185 | |
dc.identifier | 000357454200001 | |
dc.identifier.citation | Li , X , Gu , Y , Dong , H , Wang , W & Dong , C 2015 , ' Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane ' , Scientific Reports , vol. 5 , 11883 . https://doi.org/10.1038/srep11883 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/10023/7019 | |
dc.description | C.D. is a recipient of the Wellcome Trust Investigator award (WT106121MA). W.W. is supported by Science and Technology Program of Guangzhou, China (201510010040). | en |
dc.description.abstract | Lipopolysaccharide (LPS) is a main component of the outer membrane of Gram-negative bacteria, which is essential for the vitality of most Gram-negative bacteria and plays a critical role for drug resistance. LptD/E complex forms a N-terminal LPS transport slide, a hydrophobic intramembrane hole and the hydrophilic channel of the barrel, for LPS transport, lipid A insertion and core oligosaccharide and O-antigen polysaccharide translocation, respectively. However, there is no direct evidence to confirm that LptD/E transports LPS from the periplasm to the external leaflet of the outer membrane. By replacing LptD residues with an unnatural amino acid p-benzoyl-L-phenyalanine (pBPA) and UV-photo-cross-linking in E.coli, the translocon and LPS intermediates were obtained at the N-terminal domain, the intramembrane hole, the lumenal gate, the lumen of LptD channel, and the extracellular loop 1 and 4, providing the first direct evidence and "snapshots" to reveal LPS translocation steps across the outer membrane. | |
dc.format.extent | 1124229 | |
dc.language.iso | eng | |
dc.relation.ispartof | Scientific Reports | en |
dc.subject | QD Chemistry | en |
dc.subject | NDAS | en |
dc.subject.lcc | QD | en |
dc.title | Trapped lipopolysaccharide and LptD intermediates reveal lipopolysaccharide translocation steps across the Escherichia coli outer membrane | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.identifier.doi | 10.1038/srep11883 | |
dc.description.status | Peer reviewed | en |
dc.identifier.url | http://www.nature.com/articles/srep11883#supplementary-information | en |
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