Show simple item record

Files in this item


Item metadata

dc.contributor.authorAllwood, J. William
dc.contributor.authorChandra, Surya
dc.contributor.authorXu, Yun
dc.contributor.authorDunn, Warwick B.
dc.contributor.authorCorrea, Elon
dc.contributor.authorHopkins, Laura
dc.contributor.authorGoodacre, Royston
dc.contributor.authorTobin, A.K.
dc.contributor.authorBowsher, Caroline G.
dc.identifier.citationAllwood , J W , Chandra , S , Xu , Y , Dunn , W B , Correa , E , Hopkins , L , Goodacre , R , Tobin , A K & Bowsher , C G 2015 , ' Profiling of spatial metabolite distributions in wheat leaves under normal and nitrate limiting conditions ' , Phytochemistry , vol. 115 , pp. 99-111 .
dc.identifier.otherPURE: 173636331
dc.identifier.otherPURE UUID: 507493f8-f554-4581-bdba-e94c7f635c89
dc.identifier.otherScopus: 84923521685
dc.identifier.otherWOS: 000357237900009
dc.descriptionJ.W.A. and R.G. would like to thank the Sixth Framework Programme (META-PHOR – FOOD-CT-2006-036220), E.C. and R.G. the Seventh Framework Programme (CommonSense project – SEC-2010.1.3-3 ref: 261809), R.G. and W.B.D. The UK BBSRC and EPSRC (BBC0082191) for financial support of the MCISB, L.H. was funded by a NERC studentship.en
dc.description.abstractThe control and interaction between nitrogen and carbon assimilatory pathways is essential in both photosynthetic and non-photosynthetic tissue in order to support metabolic processes without compromising growth. Physiological differences between the basal and mature region of wheat (Triticum aestivum) primary leaves confirmed that there was a change from heterotrophic to autotrophic metabolism. Fourier Transform Infrared (FT-IR) spectroscopy confirmed the suitability and phenotypic reproducibility of the leaf growth conditions. Principal Component-Discriminant Function Analysis (PC-DFA) revealed distinct clustering between base, and tip sections of the developing wheat leaf, and from plants grown in the presence or absence of nitrate. Gas Chromatography-Time of Flight/Mass Spectrometry (GC-TOF/MS) combined with multivariate and univariate analyses, and Bayesian network (BN) analysis, distinguished different tissues and confirmed the physiological switch from high rates of respiration to photosynthesis along the leaf. The operation of nitrogen metabolism impacted on the levels and distribution of amino acids, organic acids and carbohydrates within the wheat leaf. In plants grown in the presence of nitrate there was reduced levels of a number of sugar metabolites in the leaf base and an increase in maltose levels, possibly reflecting an increase in starch turnover. The value of using this combined metabolomics analysis for further functional investigations in the future are discussed.
dc.rightsCopyright 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (
dc.subjectTriticum aestivumen
dc.subjectMetabolite fingerprintingen
dc.subjectMetabolite profilingen
dc.subjectBayesian network analysisen
dc.subjectQH301 Biologyen
dc.subjectQD Chemistryen
dc.titleProfiling of spatial metabolite distributions in wheat leaves under normal and nitrate limiting conditionsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.description.statusPeer revieweden

This item appears in the following Collection(s)

Show simple item record