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Intracellular zinc modulates cardiac ryanodine receptor-mediated calcium release
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dc.contributor.author | Woodier, Jason | |
dc.contributor.author | Rainbow, Richard D. | |
dc.contributor.author | Stewart, Alan J. | |
dc.contributor.author | Pitt, Samantha Jane | |
dc.date.accessioned | 2015-07-13T11:40:04Z | |
dc.date.available | 2015-07-13T11:40:04Z | |
dc.date.issued | 2015-07-10 | |
dc.identifier.citation | Woodier , J , Rainbow , R D , Stewart , A J & Pitt , S J 2015 , ' Intracellular zinc modulates cardiac ryanodine receptor-mediated calcium release ' , Journal of Biological Chemistry , vol. 290 , no. 28 , pp. 17599-17610 . https://doi.org/10.1074/jbc.M115.661280 | en |
dc.identifier.issn | 0021-9258 | |
dc.identifier.other | PURE: 192888623 | |
dc.identifier.other | PURE UUID: 0c0b7e5a-c007-4527-9ad2-ca865f9be378 | |
dc.identifier.other | Scopus: 84940093431 | |
dc.identifier.other | ORCID: /0000-0003-4580-1840/work/60195800 | |
dc.identifier.other | ORCID: /0000-0003-2257-1595/work/60196230 | |
dc.identifier.other | WOS: 000357730900050 | |
dc.identifier.uri | https://hdl.handle.net/10023/6972 | |
dc.description | This work was supported by University of St. Andrews, Tenovus Scotland Grant T14/35 (to S. J. P.), British Heart Foundation Grant FS/14/69/31001 (to S. J. P.), a grant from the John and Lucille van Geest Cardiovascular Diseases Research Fund (to the University of Leicester and R. D. R.), and Biotechnology and Biological Sciences Research Council Grant BB/J006467/1 (to A. J. S.). | en |
dc.description.abstract | Aberrant Zn2+-homeostasis is a hallmark of certain cardiomyopathies associated with altered contractile force. In this study we addressed whether Zn2+ modulates cardiac ryanodine receptor gating and Ca2+-dynamics in isolated cardiomyocytes. We reveal that Zn2+ is a high affinity regulator of RyR2 displaying three modes of operation. Picomolar free Zn2+ concentrations potentiate RyR2 responses but channel activation is still dependent on the presence of cytosolic Ca2+. At concentrations of free Zn2+ >1 nM, Zn2+ is the main activating ligand and the dependency on Ca2+ is removed. Zn2+ is therefore a higher affinity activator of RyR2 than Ca2+. Millimolar levels of free Zn2+ were found to inhibit channel openings. In cardiomyocytes, consistent with our single-channel results, we show that Zn2+ modulates both the frequency and amplitude of Ca2+ waves in a concentration dependent manner and that physiological levels of Zn2+ elicit Ca2+-release in the absence of activating levels of cytosolic Ca2+. This highlights a new role for intracellular Zn2+ in shaping Ca2+-dynamics in cardiomyocytes through modulation of RyR2 gating. | |
dc.format.extent | 12 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Biological Chemistry | en |
dc.rights | Copyright © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Author’s Choice—Final version free via Creative Commons CC-BY license. | en |
dc.subject | Ryanodine receptor | en |
dc.subject | Excitation-contraction coupling | en |
dc.subject | Calcium | en |
dc.subject | Zinc | en |
dc.subject | Heart failure | en |
dc.subject | QD Chemistry | en |
dc.subject | RC Internal medicine | en |
dc.subject | NDAS | en |
dc.subject | BDC | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | RC | en |
dc.title | Intracellular zinc modulates cardiac ryanodine receptor-mediated calcium release | en |
dc.type | Journal article | en |
dc.contributor.sponsor | BBSRC | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1074/jbc.M115.661280 | |
dc.description.status | Peer reviewed | en |
dc.identifier.grantnumber | BB/J006467/1 | en |
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