Show simple item record

Files in this item

Thumbnail

Item metadata

dc.contributor.authorYang, Zhengyi
dc.contributor.authorPiksarv, Peeter
dc.contributor.authorFerrier, David Ellard Keith
dc.contributor.authorGunn-Moore, Frank J
dc.contributor.authorDholakia, Kishan
dc.date.accessioned2015-07-10T09:10:03Z
dc.date.available2015-07-10T09:10:03Z
dc.date.issued2015-08-01
dc.identifier199933956
dc.identifierff36ab7e-4e33-4417-b4e4-ba7886bdbb5a
dc.identifier84945905586
dc.identifier000359006000005
dc.identifier.citationYang , Z , Piksarv , P , Ferrier , D E K , Gunn-Moore , F J & Dholakia , K 2015 , ' Macro-optical trapping for sample confinement in light sheet microscopy ' , Biomedical Optics Express , vol. 6 , no. 8 , pp. 2778-2785 . https://doi.org/10.1364/BOE.6.002778en
dc.identifier.issn2156-7085
dc.identifier.otherORCID: /0000-0003-3247-6233/work/36423815
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730420
dc.identifier.urihttps://hdl.handle.net/10023/6957
dc.descriptionThe authors thank the UK Engineering and Physical Sciences Research Council (EPSRC) (grant number EP/J01771X/1), Estonian Research Council (grant number PUTJD8), the RS Macdonald Charitable Trust and the ’BRAINS’ 600th Anniversary appeal for funding.en
dc.description.abstractLight sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrate an integrated light sheet microscope which solely uses optical forces to trap and hold the sample using a counter-propagating laser beam geometry. Specifically, tobacco plant cells and living Spirobranchus lamarcki larvae were successfully trapped and sectional images acquired. This novel approach has the potential to significantly expand the range of applications for light sheet imaging.
dc.format.extent1387226
dc.language.isoeng
dc.relation.ispartofBiomedical Optics Expressen
dc.subjectQC Physicsen
dc.subjectQR Microbiologyen
dc.subjectDASen
dc.subject.lccQCen
dc.subject.lccQRen
dc.titleMacro-optical trapping for sample confinement in light sheet microscopyen
dc.typeJournal articleen
dc.contributor.sponsorEPSRCen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews. Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews. Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.identifier.doi10.1364/BOE.6.002778
dc.description.statusPeer revieweden
dc.identifier.urlhttps://www.osapublishing.org/boe/fulltext.cfm?uri=boe-6-8-2778&id=321741#articleSupplMaten
dc.identifier.grantnumberEP/J01771X/1en


This item appears in the following Collection(s)

Show simple item record