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dc.contributor.authorKoussounadis, Antonis
dc.contributor.authorLangdon, Simon
dc.contributor.authorUm, In Hwa
dc.contributor.authorHarrison, David James
dc.contributor.authorSmith, V Anne
dc.date.accessioned2015-07-08T13:17:38Z
dc.date.available2015-07-08T13:17:38Z
dc.date.issued2015-06-08
dc.identifier.citationKoussounadis , A , Langdon , S , Um , I H , Harrison , D J & Smith , V A 2015 , ' Relationship between differentially expressed mRNA and mRNA-protein correlations in a xenograft model system ' , Scientific Reports , vol. 5 , 10775 . https://doi.org/10.1038/srep10775en
dc.identifier.issn2045-2322
dc.identifier.otherPURE: 184482238
dc.identifier.otherPURE UUID: 7a40ab49-2afe-4ad5-a1c8-df6fd79e991a
dc.identifier.otherScopus: 84930646796
dc.identifier.otherORCID: /0000-0002-0487-2469/work/32209212
dc.identifier.otherWOS: 000356137300001
dc.identifier.otherORCID: /0000-0001-9041-9988/work/64034301
dc.identifier.urihttp://hdl.handle.net/10023/6931
dc.descriptionThis work was supported by Medical Research Scotland (FRG353 to VAS), the FP7- Directorate-General for Research and Innovation of the European Commission (EU HEALTHF4-2012-305033 to Coordinating Action Systems Medicine to DJH); the Chief Scientist Office of Scotland (to DJH) and the Scottish Funding Council (to DJH and SPL).en
dc.description.abstractDifferential mRNA expression studies implicitly assume that changes in mRNA expression have biological meaning, most likely mediated by corresponding changes in protein levels. Yet studies into mRNA-protein correspondence have shown notoriously poor correlation between mRNA and protein expression levels, creating concern for inferences from only mRNA expression data. However, none of these studies have examined in particular differentially expressed mRNA. Here, we examined this question in an ovarian cancer xenograft model. We measured protein and mRNA expression for twenty-nine genes in four drug-treatment conditions and in untreated controls. We identified mRNAs differentially expressed between drug-treated xenografts and controls, then analysed mRNA-protein expression correlation across a five-point time-course within each of the four experimental conditions. We evaluated correlations between mRNAs and their protein products for mRNAs differentially expressed within an experimental condition compared to those that are not. We found that differentially expressed mRNAs correlate significantly better with their protein product than non-differentially expressed mRNAs. This result increases confidence for the use of differential mRNA expression for biological discovery in this system, as well as providing optimism for the usefulness of inferences from mRNA expression in general.
dc.format.extent9
dc.language.isoeng
dc.relation.ispartofScientific Reportsen
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/en
dc.subjectQH301 Biologyen
dc.subjectBDCen
dc.subject.lccQH301en
dc.titleRelationship between differentially expressed mRNA and mRNA-protein correlations in a xenograft model systemen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Scottish Oceans Instituteen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Centre for Research into Ecological & Environmental Modellingen
dc.contributor.institutionUniversity of St Andrews.Centre for Biological Diversityen
dc.identifier.doihttps://doi.org/10.1038/srep10775
dc.description.statusPeer revieweden


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