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dc.contributor.authorJamieson, L.E.
dc.contributor.authorHarrison, David James
dc.contributor.authorCampbell, C.J.
dc.date.accessioned2015-06-10T10:40:03Z
dc.date.available2015-06-10T10:40:03Z
dc.date.issued2015-06-21
dc.identifier193150012
dc.identifier1ecce922-25ab-46a1-a697-79b51a31f750
dc.identifier84930747721
dc.identifier000355852400004
dc.identifier.citationJamieson , L E , Harrison , D J & Campbell , C J 2015 , ' Chemical analysis of multicellular tumour spheroids ' , Analyst , vol. 140 , no. 12 , pp. 3910-3920 . https://doi.org/10.1039/c5an00524hen
dc.identifier.issn0003-2654
dc.identifier.otherORCID: /0000-0001-9041-9988/work/64034354
dc.identifier.urihttps://hdl.handle.net/10023/6794
dc.descriptionThis research received support from the QNano Project http://www.qnano-ri.eu which is financed by the European Community Research Infrastructures under the FP7 Capacities Programme (grant no. INFRA-2010-262163), and its partner Trinity College Dublin.en
dc.description.abstractConventional two dimensional (2D) monolayer cell culture has been considered the ‘gold standard’ technique for in vitro cellular experiments. However, the need for a model that better mimics the three dimensional (3D) architecture of tissue in vivo has led to the development of Multicellular Tumour Spheroids (MTS) as a 3D tissue culture model. To some extent MTS mimic the environment of in vivo tumours where, for example, oxygen and nutrient gradients develop, protein expression changes and cells form a spherical structure with regions of proliferation, senescence and necrosis. This review focuses on the development of techniques for chemical analysis of MTS as a tool for understanding in vivo tumours and a platform for more effective drug and therapy discovery. While traditional monolayer techniques can be translated to 3D models, these often fail to provide the desired spatial resolution and z-penetration for live cell imaging. More recently developed techniques for overcoming these problems will be discussed with particular reference to advances in instrument technology for achieving the increased spatial resolution and imaging depth required.
dc.format.extent2247700
dc.language.isoeng
dc.relation.ispartofAnalysten
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.subjectT-NDASen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.subject.lccRC0254en
dc.titleChemical analysis of multicellular tumour spheroidsen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.identifier.doihttps://doi.org/10.1039/c5an00524h
dc.description.statusPeer revieweden


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