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dc.contributor.authorChen, Mingzhou
dc.contributor.authorMcReynolds, Naomi
dc.contributor.authorCampbell, Elaine Catherine
dc.contributor.authorMazilu, Michael
dc.contributor.authorBarbosa, Joao
dc.contributor.authorDholakia, Kishan
dc.contributor.authorPowis, Simon John
dc.identifier.citationChen , M , McReynolds , N , Campbell , E C , Mazilu , M , Barbosa , J , Dholakia , K & Powis , S J 2015 , ' The use of Wavelength Modulated Raman Spectroscopy in label-free identification of T lymphocyte subsets, Natural Killer cells and Dendritic cells ' , PLoS One , vol. 10 , no. 5 , e0125158 .
dc.identifier.otherPURE: 179438713
dc.identifier.otherPURE UUID: cb9837b0-4787-4304-9cab-536d65fa418f
dc.identifier.otherScopus: 84930620601
dc.identifier.otherORCID: /0000-0002-6190-5167/work/47136399
dc.identifier.otherORCID: /0000-0003-4218-2984/work/60195330
dc.identifier.otherWOS: 000354921400025
dc.descriptionThis work was funded by a Cancer Research United Kingdom, Engineering and Physical Sciences Research Council (grant EP/J01771X/1), Medical Research Council and Department of Health England Imaging Programme (MC, MM KD), and by A European Union FAMOS project (FP7 ICT, 317744) to KD.en
dc.description.abstractDetermining the identity of cells of the immune system usually involves destructive fixation and chemical staining, or labeling with fluorescently labeled antibodies recognising specific cell surface markers. Completely label-free identification would be a significant advantage in conditions where untouched cells are a priority. We demonstrate here the use of Wavelength Modulated Raman Spectroscopy, to achieve label-free identification of purified, unfixed and untouched populations of major immune cell subsets isolated from healthy human donors. Using this technique we have been able to distinguish between CD4+ T lymphocytes, CD8+ T lymphocytes and CD56+ Natural Killer cells at specificities of up to 96%. Additionally, we have been able to distinguish between CD303+ plasmacytoid and CD1c+ myeloid dendritic cell subsets, the key initiator and regulatory cells of many immune responses. This demonstrates the ability to identify unperturbed cells of the immune system, and opens novel opportunities to analyse immunological systems and to develop fully label-free diagnostic technologies.
dc.relation.ispartofPLoS Oneen
dc.rights© 2015 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.subjectRaman spectroscopyen
dc.subjectDendritic cellsen
dc.subjectNatural killer cellsen
dc.subjectT Lymphocytesen
dc.subjectR Medicineen
dc.subjectQC Physicsen
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleThe use of Wavelength Modulated Raman Spectroscopy in label-free identification of T lymphocyte subsets, Natural Killer cells and Dendritic cellsen
dc.typeJournal articleen
dc.contributor.sponsorEuropean Commissionen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.contributor.institutionUniversity of St Andrews. Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews. School of Physics and Astronomyen
dc.description.statusPeer revieweden

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