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dc.contributor.authorCavanagh, Jorunn Pauline
dc.contributor.authorHjerde, Erik
dc.contributor.authorHolden, Matthew T G
dc.contributor.authorKahlke, Tim
dc.contributor.authorKlingenberg, Claus
dc.contributor.authorFlægstad, Trond
dc.contributor.authorParkhill, Julian
dc.contributor.authorBentley, Stephen D
dc.contributor.authorSollid, Johanna U Ericson
dc.date.accessioned2015-05-25T13:40:01Z
dc.date.available2015-05-25T13:40:01Z
dc.date.issued2014-11-11
dc.identifier.citationCavanagh , J P , Hjerde , E , Holden , M T G , Kahlke , T , Klingenberg , C , Flægstad , T , Parkhill , J , Bentley , S D & Sollid , J U E 2014 , ' Whole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitals ' , Journal of Antimicrobial Chemotherapy , vol. 69 , no. 11 , pp. 2920-2927 . https://doi.org/10.1093/jac/dku271en
dc.identifier.issn0305-7453
dc.identifier.otherPURE: 138013240
dc.identifier.otherPURE UUID: bf8f05d0-fb94-43ff-b099-812031b75274
dc.identifier.otherPubMed: 25038069
dc.identifier.otherScopus: 84928194489
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196469
dc.identifier.urihttp://hdl.handle.net/10023/6688
dc.description.abstractObjectives Staphylococcus haemolyticus is an emerging cause of nosocomial infections, primarily affecting immunocompromised patients. A comparative genomic analysis was performed on clinical S. haemolyticus isolates to investigate their genetic relationship and explore the coding sequences with respect to antimicrobial resistance determinants and putative hospital adaptation. Methods Whole-genome sequencing was performed on 134 isolates of S. haemolyticus from geographically diverse origins (Belgium, 2; Germany, 10; Japan, 13; Norway, 54; Spain, 2; Switzerland, 43; UK, 9; USA, 1). Each genome was individually assembled. Protein coding sequences (CDSs) were predicted and homologous genes were categorized into three types: Type I, core genes, homologues present in all strains; Type II, unique core genes, homologues shared by only a subgroup of strains; and Type III, unique genes, strain-specific CDSs. The phylogenetic relationship between the isolates was built from variable sites in the form of single nucleotide polymorphisms (SNPs) in the core genome and used to construct a maximum likelihood phylogeny. Results SNPs in the genome core regions divided the isolates into one major group of 126 isolates and one minor group of isolates with highly diverse genomes. The major group was further subdivided into seven clades (A–G), of which four (A–D) encompassed isolates only from Europe. Antimicrobial multiresistance was observed in 77.7% of the collection. High levels of homologous recombination were detected in genes involved in adherence, staphylococcal host adaptation and bacterial cell communication. Conclusions The presence of several successful and highly resistant clones underlines the adaptive potential of this opportunistic pathogen.
dc.language.isoeng
dc.relation.ispartofJournal of Antimicrobial Chemotherapyen
dc.rights© The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectStaphylococcien
dc.subjectSCCmecen
dc.subjectMolecular epidemiologyen
dc.subjectMultidrug resistanceen
dc.subjectBacterial genomicsen
dc.subjectRA0421 Public health. Hygiene. Preventive Medicineen
dc.subject.lccRA0421en
dc.titleWhole-genome sequencing reveals clonal expansion of multiresistant Staphylococcus haemolyticus in European hospitalsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1093/jac/dku271
dc.description.statusPeer revieweden
dc.identifier.urlhttp://jac.oxfordjournals.org/content/69/11/2920/suppl/DC1en


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