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Unraveling the specific regulation of the mdb central pathway for the anaerobic degradation of 3-methylbenzoate

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Date
08/05/2015
Author
Juárez, Javier F
Liu, Huixiang
Zamarro, Maria T
McMahon, Stephen
Liu, Huanting
Naismith, Jim
Eberlein, Christian
Boll, Matthias
Carmona, Manuel
Diaz, Eduardo
Funder
BBSRC
BBSRC
Grant ID
BB/K015508/1
BB/K015508/1
Keywords
Transcriptional regulation
Azoarcus
Anaerobic degradation
3-methylbenzoate
QD Chemistry
DAS
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Abstract
The mbd cluster encodes the anaerobic degradation of 3-methylbenzoate in the β-proteobacterium Azoarcus sp. CIB. The specific transcriptional regulation circuit that controls the expression of the mbd genes was investigated. The PO, PB1, and P3R promoters responsible for the expression of the mbd genes, their cognate MbdR transcriptional repressor, as well as the MbdR operator regions (ATACN10GTAT) have been characterized. The three-dimensional structure of MbdR has been solved revealing a conformation similar to that of other TetR family transcriptional regulators. The first intermediate of the catabolic pathway, i.e. 3-methylbenzoyl-CoA, was shown to act as the inducer molecule. An additional MbdR-dependent promoter, PA, which contributes to the expression of the CoA ligase that activates 3-methylbenzoate to 3-methylbenzoyl-CoA, was shown to be necessary for an efficient induction of the mbd genes. Our results suggest that the mbd cluster recruited a regulatory system based on the MbdR regulator and its target promoters to evolve a distinct central catabolic pathway that is only expressed for the anaerobic degradation of aromatic compounds that generate 3-methylbenzoyl-CoA as the central metabolite. All these results highlight the importance of the regulatory systems in the evolution and adaptation of bacteria to the anaerobic degradation of aromatic compounds.
Citation
Juárez , J F , Liu , H , Zamarro , M T , McMahon , S , Liu , H , Naismith , J , Eberlein , C , Boll , M , Carmona , M & Diaz , E 2015 , ' Unraveling the specific regulation of the mdb central pathway for the anaerobic degradation of 3-methylbenzoate ' , Journal of Biological Chemistry , vol. 290 , pp. 12165-12183 . https://doi.org/10.1074/jbc.M115.637074
Publication
Journal of Biological Chemistry
Status
Peer reviewed
DOI
https://doi.org/10.1074/jbc.M115.637074
ISSN
0021-9258
Type
Journal article
Rights
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc. Final version free via Creative Commons CC-BY license (http://creativecommons.org/licenses/by/3.0)
Description
This work was supported by Ministry of Economy and Competitiveness of Spain Grants BIO2009-10438; BIO2012-39501, CSD2007-00005; European Union FP7 Grant 311815. Crystallography was supported by a BBSRC grant and Wellcome Trust award.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/6675

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