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dc.contributor.authorFortunato, Sofia
dc.contributor.authorAdamski, Marcin
dc.contributor.authorMendivil Ramos, Olivia
dc.contributor.authorLeininger, Sven
dc.contributor.authorLiu, Jing
dc.contributor.authorFerrier, David Ellard Keith
dc.contributor.authorAdamska, Maja
dc.identifier.citationFortunato , S , Adamski , M , Mendivil Ramos , O , Leininger , S , Liu , J , Ferrier , D E K & Adamska , M 2014 , ' Calcisponges have a ParaHox gene and dynamic expression of dispersed NK homeobox genes ' , Nature , vol. 514 , no. 7524 , pp. 620-623 .
dc.identifier.otherORCID: /0000-0003-3247-6233/work/36423819
dc.descriptionThis study was funded by the Sars Centre core budget to M. Adamska. Sequencing was performed at the Norwegian High Throughput Sequencing Centre funded by the Norwegian Research Council. O.M.R. and D.E.K.F. acknowledge support from the BBSRC and the School of Biology, University of St Andrews.en
dc.description.abstractSponges are simple animals with few cell types, but their genomes paradoxically contain a wide variety of developmental transcription factors1,2,3,4, including homeobox genes belonging to the Antennapedia (ANTP) class5,6, which in bilaterians encompass Hox, ParaHox and NK genes. In the genome of the demosponge Amphimedon queenslandica, no Hox or ParaHox genes are present, but NK genes are linked in a tight cluster similar to the NK clusters of bilaterians5. It has been proposed that Hox and ParaHox genes originated from NK cluster genes after divergence of sponges from the lineage leading to cnidarians and bilaterians5,7. On the other hand, synteny analysis lends support to the notion that the absence of Hox and ParaHox genes in Amphimedon is a result of secondary loss (the ghost locus hypothesis)8. Here we analysed complete suites of ANTP-class homeoboxes in two calcareous sponges, Sycon ciliatum and Leucosolenia complicata. Our phylogenetic analyses demonstrate that these calcisponges possess orthologues of bilaterian NK genes (Hex, Hmx and Msx), a varying number of additional NK genes and one ParaHox gene, Cdx. Despite the generation of scaffolds spanning multiple genes, we find no evidence of clustering of Sycon NK genes. All Sycon ANTP-class genes are developmentally expressed, with patterns suggesting their involvement in cell type specification in embryos and adults, metamorphosis and body plan patterning. These results demonstrate that ParaHox genes predate the origin of sponges, thus confirming the ghost locus hypothesis8, and highlight the need to analyse the genomes of multiple sponge lineages to obtain a complete picture of the ancestral composition of the first animal genome.
dc.subjectQH301 Biologyen
dc.titleCalcisponges have a ParaHox gene and dynamic expression of dispersed NK homeobox genesen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.contributor.institutionUniversity of St Andrews. Marine Alliance for Science & Technology Scotlanden
dc.contributor.institutionUniversity of St Andrews. Scottish Oceans Instituteen
dc.description.statusPeer revieweden

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