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Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers
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dc.contributor.author | Forrest, S. | |
dc.contributor.author | Lear, Z. | |
dc.contributor.author | Herod, M.R. | |
dc.contributor.author | Ryan, M. | |
dc.contributor.author | Rowlands, D.J. | |
dc.contributor.author | Stonehouse, N.J. | |
dc.date.accessioned | 2015-04-20T11:01:01Z | |
dc.date.available | 2015-04-20T11:01:01Z | |
dc.date.issued | 2014-12-01 | |
dc.identifier.citation | Forrest , S , Lear , Z , Herod , M R , Ryan , M , Rowlands , D J & Stonehouse , N J 2014 , ' Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers ' , Journal of General Virology , vol. 95 , pp. 2649-2657 . https://doi.org/10.1099/vir.0.067751-0 | en |
dc.identifier.issn | 0022-1317 | |
dc.identifier.other | PURE: 159067485 | |
dc.identifier.other | PURE UUID: afbcc76e-d570-44d4-a5e7-c2af874eace7 | |
dc.identifier.other | Scopus: 84910637952 | |
dc.identifier.other | WOS: 000350943800007 | |
dc.identifier.other | PubMed: 25096816 | |
dc.identifier.other | ORCID: /0000-0002-0012-0614/work/47136065 | |
dc.identifier.uri | https://hdl.handle.net/10023/6521 | |
dc.description | Sophie Forrest was funded by a BBSRC studentship and by the Wellcome Trust (094898). Zoe Lear is funded by a Wellcome Trust 4-year PhD programme (The Molecular Basis of Biological Mechanisms). | en |
dc.description.abstract | We have previously documented the inhibitory activity of RNA aptamers to the RNA-dependent RNA polymerase of foot-and-mouth disease virus (3D). Here we report their modification and use with a subgenomic replicon incorporating GFP (pGFP-PAC replicon), allowing replication to be monitored and quantified in real-time. GFP expression in transfected BHK-21 cells reached a maximum at approximately 8 h post-transfection, at which time change in morphology of the cells was consistent with a virus-induced cytopathic effect. However, transfection of replicon-bearing cells with a 3D aptamer RNA resulted in inhibition of GFP expression and maintenance of normal cell morphology, whereas a control aptamer RNA had little effect. The inhibition was correlated with a reduction in 3D (detected by immunoblotting) and shown to be dose dependent. The 3D aptamers appeared to be more effective than 29-C-methylcytidine (29CMC). Aptamers to components of the replication complex are therefore useful molecular tools for studying viral replication and also have potential as diagnostic molecules in the future. | |
dc.format.extent | 9 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of General Virology | en |
dc.rights | Copyright 2014 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.subject | QR355 Virology | en |
dc.subject.lcc | QR355 | en |
dc.title | Inhibition of the foot-and-mouth disease virus subgenomic replicon by RNA aptamers | en |
dc.type | Journal article | en |
dc.contributor.sponsor | BBSRC | en |
dc.description.version | Publisher PDF | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | https://doi.org/10.1099/vir.0.067751-0 | |
dc.description.status | Peer reviewed | en |
dc.identifier.url | http://vir.sgmjournals.org/content/95/Pt_12/2649/suppl/DC1 | en |
dc.identifier.grantnumber | BB/K003801/1 | en |
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