Reproducible surface-enhanced Raman quantification of biomarkers in multicomponent mixtures
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Direct and quantitative detection of unlabeled glycerophosphoinositol (GroPIns), an abundant cytosolic phosphoinositide derivative, would allow rapid evaluation of several malignant cell transformations. Here we report label-free analysis of GroPIns via surface-enhanced Raman spectroscopy (SERS) with a sensitivity of 200 nM, well below its apparent concentration in cells. Crucially, our SERS substrates, based on lithographically defined gold nanofeatures, can be used to predict accurately the GroPIns concentration even in multicomponent mixtures, avoiding the preliminary separation of individual compounds. Our results represent a critical step toward the creation of SERS-based biosensor for rapid, label-free, and reproducible detection of specific molecules, overcoming limits of current experimental methods.
De Luca , A C , Reader-Harris , P , Mazilu , M , Mariggio , S , Corda , D & Di Falco , A 2014 , ' Reproducible surface-enhanced Raman quantification of biomarkers in multicomponent mixtures ' ACS Nano , vol 8 , no. 3 , pp. 2575-2583 . DOI: 10.1021/nn406200y
This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Nano, copyright © 2014 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/nn406200y.
A.C.D.L. is supported by an AIRC Start-up Grant 11454 and a FIR project RBFR12WAPY. A.D.F. is supported by an EPSRC Career Acceleration Fellowship (EP/I004602/1). D.C. is supported by an AIRC Grant IG10341 and the projects PON01-00117 and PON01-00862. S.M. is supported by a PRIN project 2012CK5RPF_05.
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