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dc.contributor.authorRockenfeller, P.
dc.contributor.authorKoska, M.
dc.contributor.authorPietrocola, F.
dc.contributor.authorMinois, N.
dc.contributor.authorKnittelfelder, O.
dc.contributor.authorSica, V.
dc.contributor.authorFranz, J.
dc.contributor.authorCarmona-Gutierrez, D.
dc.contributor.authorKroemer, G.
dc.contributor.authorMadeo, F.
dc.date.accessioned2015-03-16T17:01:08Z
dc.date.available2015-03-16T17:01:08Z
dc.date.issued2015-03
dc.identifier.citationRockenfeller , P , Koska , M , Pietrocola , F , Minois , N , Knittelfelder , O , Sica , V , Franz , J , Carmona-Gutierrez , D , Kroemer , G & Madeo , F 2015 , ' Phosphatidylethanolamine positively regulates autophagy and longevity ' Cell death and differentiation , vol 22 , no. 3 , pp. 499-508 . DOI: 10.1038/cdd.2014.219en
dc.identifier.issn1350-9047
dc.identifier.otherPURE: 174965088
dc.identifier.otherPURE UUID: 6b77822a-352b-499d-bbee-1b8832530d2e
dc.identifier.otherWOS: 000349543600014
dc.identifier.urihttp://hdl.handle.net/10023/6250
dc.descriptionThis work was supported by the Austrian Science Fund FWF (grants LIPOTOX, I1000-B20, P23490-B12, and P24381-B20) to FM. The authors acknowledge support from NAWI Graz. OK is a member of the PhD program ‘Molecular Enzymology’, funded by the FWF (project W901-B12). GK is supported by the Ligue contre le Cancer (équipe labelisée); Agence National de la Recherche (ANR); Association pour la recherche sur le cancer (ARC); Cancéropôle Ile-de-France; Institut National du Cancer (INCa); Fondation Bettencourt-Schueller; Fondation de France; Fondation pour la Recherche Médicale (FRM); the European Commission (ArtForce); the European Research Council (ERC); the LabEx Immuno-Oncology; the SIRIC Stratified Oncology Cell DNA Repair and Tumour Immune Elimination (SOCRATE); the SIRIC Cancer Research and Personalized Medicine (CARPEM); and the Paris Alliance of Cancer Research Institutes (PACRI).en
dc.description.abstractAutophagy is a cellular recycling program that retards ageing by efficiently eliminating damaged and potentially harmful organelles and intracellular protein aggregates. Here, we show that the abundance of phosphatidylethanolamine (PE) positively regulates autophagy. Reduction of intracellular PE levels by knocking out either of the two yeast phosphatidylserine decarboxylases (PSD) accelerated chronological ageing-associated production of reactive oxygen species and death. Conversely, the artificial increase of intracellular PE levels, by provision of its precursor ethanolamine or by overexpression of the PE-generating enzyme Psd1, significantly increased autophagic flux, both in yeast and in mammalian cell culture. Importantly administration of ethanolamine was sufficient to extend the lifespan of yeast (Saccharomyces cerevisiae), mammalian cells (U2OS, H4) and flies (Drosophila melanogaster). We thus postulate that the availability of PE may constitute a bottleneck for functional autophagy and that organismal life or healthspan could be positively influenced by the consumption of ethanolamine-rich food.en
dc.format.extent10en
dc.language.isoeng
dc.relation.ispartofCell death and differentiationen
dc.rightsCopyright 2015 the Authors. This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/.en
dc.subjectYeast saccharomyces-cerevisiaeen
dc.subjectLipid droplet formationen
dc.subjectPhosphatidylserine decarboxylaseen
dc.subjectSubcellular membranesen
dc.subjectMonitoring autophagyen
dc.subjectProtein lipidationen
dc.subjectDiffernet pathwaysen
dc.subjectMammalian-cellsen
dc.subjectLife-spanen
dc.subjectGeneen
dc.subjectQR Microbiologyen
dc.subject.lccQRen
dc.titlePhosphatidylethanolamine positively regulates autophagy and longevityen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Biologyen
dc.identifier.doihttp://dx.doi.org/10.1038/cdd.2014.219
dc.description.statusPeer revieweden


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