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dc.contributor.authorBader, S A
dc.contributor.authorWalker, M
dc.contributor.authorHarrison, D J
dc.identifier.citationBader , S A , Walker , M & Harrison , D J 2007 , ' A human cancer-associated truncation of MBD4 causes dominant negative impairment of DNA repair in colon cancer cells ' , British Journal of Cancer , vol. 96 , no. 4 , pp. 660-666 .
dc.identifier.otherRIS: urn:C1E1695C59011939EA928E18E1C72E77
dc.identifier.otherORCID: /0000-0001-9041-9988/work/64034284
dc.description.abstractMBD4 binds to methylated DNA and acts as a thymine DNA glycosylase in base excision repair. Deficiency of MBD4 in mice enhances mutation at CpG sites and alters apoptosis in response to DNA damage, but does not increase tumorigenesis in mismatch repair-deficient mice. However, in humans, frameshift mutation of MBD4, rather than deletion, is what occurs in up to 43% of microsatellite unstable colon cancers. There is no murine equivalent of this mutation. We now show that recombinant truncated MBD4 (MBD4(tru)) inhibits glycosylase activities of normal MBD4 or Uracil DNA glycosylase in cell-free assays as a dominant negative effect. Furthermore, overexpression of MBD4(tru) in Big Blue (lacI)-transfected, MSI human colorectal carcinoma cells doubled mutation frequency, indicating that the modest dominant negative effect on DNA repair can occur in living cells in short-term experiments. Intriguingly, the whole mutation spectrum was increased, not only at CpG sites, suggesting that truncated MBD4 has a more widespread effect on genomic stability. This demonstration of a dominant negative effect may be of significance in tumour progression and acquisition of drug resistance.
dc.relation.ispartofBritish Journal of Canceren
dc.subjectColorectal canceren
dc.subjectGenomic instabilityen
dc.subjectBig Blueen
dc.subjectMutation frequencyen
dc.subjectR Medicineen
dc.subjectRC0254 Neoplasms. Tumors. Oncology (including Cancer)en
dc.subjectSDG 3 - Good Health and Well-beingen
dc.titleA human cancer-associated truncation of MBD4 causes dominant negative impairment of DNA repair in colon cancer cellsen
dc.typeJournal articleen
dc.contributor.institutionUniversity of St Andrews. School of International Relationsen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.description.statusPeer revieweden

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