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dc.contributor.authorZhang, Jing
dc.contributor.authorRouillon, Christophe
dc.contributor.authorKerou, Melina
dc.contributor.authorReeks, Judith
dc.contributor.authorBrugger, Kim
dc.contributor.authorGraham, Shirley
dc.contributor.authorReimann, Julia
dc.contributor.authorCannone, Giuseppe
dc.contributor.authorLiu, Huanting
dc.contributor.authorAlbers, Sonja-Verena
dc.contributor.authorNaismith, James H.
dc.contributor.authorSpagnolo, Laura
dc.contributor.authorWhite, Malcolm F
dc.identifier.citationZhang , J , Rouillon , C , Kerou , M , Reeks , J , Brugger , K , Graham , S , Reimann , J , Cannone , G , Liu , H , Albers , S-V , Naismith , J H , Spagnolo , L & White , M F 2012 , ' Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity ' , Molecular Cell , vol. 45 , no. 3 , pp. 303-313 .
dc.identifier.otherPURE: 18094393
dc.identifier.otherPURE UUID: c54d238d-c149-4bf1-ad45-591bcd8e34ec
dc.identifier.otherWOS: 000300481100006
dc.identifier.otherScopus: 84856778250
dc.identifier.otherORCID: /0000-0003-1543-9342/work/47136090
dc.description.abstractThe prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endo-nucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5' sequence in the crRNA, but not on the presence of a proto-spacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.
dc.relation.ispartofMolecular Cellen
dc.rights(c)2012 Elsevier Inc. This article is available under an Elsevier user license which allows re-use for non-commercial purposes. See
dc.subjectQR355 Virologyen
dc.titleStructure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunityen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.School of Chemistryen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.contributor.institutionUniversity of St Andrews.EaSTCHEMen
dc.description.statusPeer revieweden

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