Cutting a fine figure : On the use of thin sections in electron microscopy to quantify autophagy
Abstract
Over the last few years, two guideline articles have been published with recommendations for assessing autophagy. These articles contained advice on quantification of autophagy by electron microscopy and proposed using thin slices for quantifying autophagic structures. Here, we expand on what can and cannot be quantified using single 2D slices and give some suggestions for efficient and minimally biased approaches for quantifying this fascinating and important process. We recommend that the journal Autophagy follow other journals in demanding stringent random sampling design and application of unbiased design-based quantification when reviewing submitted manuscripts.
Citation
Lucocq , J M & Hacker , C 2013 , ' Cutting a fine figure : On the use of thin sections in electron microscopy to quantify autophagy ' , Autophagy , vol. 9 , no. 9 , pp. 1443-1448 . https://doi.org/10.4161/auto.25570
Publication
Autophagy
Status
Peer reviewed
ISSN
1554-8635Type
Journal article
Rights
Copyright © 2013 Landes Bioscience This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
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