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Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism

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RNA_2013_Dong_1129_36.pdf (1.490Mb)
Date
08/2013
Author
Dong, Haohao
Li, Ping
Boettcher, Bettina
Elliott, Richard M.
Dong, Changjiang
Keywords
Schmallenberg virus
orthobunyavirus
bunyavirus
nucleoprotein structure
RNA replication and transcription
Virus
Encapsidation
Europe
Binding
QR355 Virology
Metadata
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Abstract
Schmallenberg virus (SBV) is a newly emerged orthobunyavirus (family Bunyaviridae) that has caused severe disease in the offspring of farm animals across Europe. Like all orthobunyaviruses, SBV contains a tripartite negative-sense RNA genome that is encapsidated by the viral nucleocapsid (N) protein in the form of a ribonucleoprotein complex (RNP). We recently reported the three-dimensional structure of SBV N that revealed a novel fold. Here we report the crystal structure of the SBV N protein in complex with a 42-nt-long RNA to 2.16 angstrom resolution. The complex comprises a tetramer of N that encapsidates the RNA as a cross-shape inside the protein ring structure, with each protomer bound to 11 ribonucleotides. Eight bases are bound in the positively charged cleft between the N- and C-terminal domains of N, and three bases are shielded by the extended N-terminal arm. SBV N appears to sequester RNA using a different mechanism compared with the nucleoproteins of other negative-sense RNA viruses. Furthermore, the structure suggests that RNA binding results in conformational changes of some residues in the RNA-binding cleft and the N- and C-terminal arms. Our results provide new insights into the novel mechanism of RNA encapsidation by orthobunyaviruses.
Citation
Dong , H , Li , P , Boettcher , B , Elliott , R M & Dong , C 2013 , ' Crystal structure of Schmallenberg orthobunyavirus nucleoprotein-RNA complex reveals a novel RNA sequestration mechanism ' , RNA , vol. 19 , no. 8 , pp. 1129-1136 . https://doi.org/10.1261/rna.039057.113
Publication
RNA
Status
Peer reviewed
DOI
https://doi.org/10.1261/rna.039057.113
ISSN
1355-8382
Type
Journal article
Rights
© 2013; Published by Cold Spring Harbor Laboratory Press for the RNA Society. This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.
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  • University of St Andrews Research
URI
http://hdl.handle.net/10023/5950

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