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dc.contributor.authorArita, Yoshihiko
dc.contributor.authorAntkowiak, Maciej
dc.contributor.authorGunn-Moore, Frank
dc.contributor.authorDholakia, Kishan
dc.contributor.editorLarin, KV
dc.contributor.editorSampson, DD
dc.identifier.citationArita , Y , Antkowiak , M , Gunn-Moore , F & Dholakia , K 2014 , Imaging the cellular response to transient shear stress using time-resolved digital holography . in KV Larin & DD Sampson (eds) , Optical Elastography and Tissue Biomechanics . vol. 8946 , Proceedings of SPIE , vol. 8946 , SPIE , Bellingham , Conference on Optical Elastography and Tissue Biomechanics , Canada , 1/02/14 .
dc.identifier.otherPURE: 148892157
dc.identifier.otherPURE UUID: b5016477-9d07-4b7f-98a6-339355f665fe
dc.identifier.otherWOS: 000334343200014
dc.identifier.otherScopus: 84897406829
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730431
dc.identifier.otherWOS: 000334343200014
dc.description.abstractShear stress has been recognized as one of the biophysical methods by which to permeabilize plasma membranes of cells. In particular, high pressure transient hydrodynamic flows created by laser-induced cavitation have been shown to lead to the uptake of fluorophores and plasmid DNA. While the mechanism and dynamics of cavitation have been extensively studied using a variety of time-resolved imaging techniques, the cellular response to the cavitation bubble and cavitation induced transient hydrodynamic flows has never been shown in detail. We use time-resolved quantitative phase microscopy to study cellular response to laser-induced cavitation bubbles. Laser-induced breakdown of an optically trapped polystyrene nanoparticle (500 nm in diameter) irradiated with a single nanosecond laser pulse at 532 nm creates transient shear stress to surrounding cells without causing cell lysis. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to laser-induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laser-induced breakdown of an optically trapped nanoparticle offers localized cavitation (70 pm in diameter), which interacts with a single cell.
dc.relation.ispartofOptical Elastography and Tissue Biomechanicsen
dc.relation.ispartofseriesProceedings of SPIEen
dc.rightsCopyright 2014 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.en
dc.subjectDigital holographyen
dc.subjectTime-resolved imagingen
dc.subjectLaser-induced breakdownen
dc.subjectOptical trappingen
dc.subjectCell transfectionen
dc.subjectMolecular deliveryen
dc.subjectQC Physicsen
dc.subjectQD Chemistryen
dc.subjectR Medicine (General)en
dc.titleImaging the cellular response to transient shear stress using time-resolved digital holographyen
dc.typeConference itemen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Physics and Astronomyen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen

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