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Imaging the cellular response to transient shear stress using time-resolved digital holography
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dc.contributor.author | Arita, Yoshihiko | |
dc.contributor.author | Antkowiak, Maciej | |
dc.contributor.author | Gunn-Moore, Frank | |
dc.contributor.author | Dholakia, Kishan | |
dc.contributor.editor | Larin, KV | |
dc.contributor.editor | Sampson, DD | |
dc.date.accessioned | 2014-09-16T16:01:08Z | |
dc.date.available | 2014-09-16T16:01:08Z | |
dc.date.issued | 2014-02-26 | |
dc.identifier | 148892157 | |
dc.identifier | b5016477-9d07-4b7f-98a6-339355f665fe | |
dc.identifier | 000334343200014 | |
dc.identifier | 84897406829 | |
dc.identifier | 000334343200014 | |
dc.identifier.citation | Arita , Y , Antkowiak , M , Gunn-Moore , F & Dholakia , K 2014 , Imaging the cellular response to transient shear stress using time-resolved digital holography . in KV Larin & DD Sampson (eds) , Optical Elastography and Tissue Biomechanics . vol. 8946 , Proceedings of SPIE , vol. 8946 , SPIE , Bellingham , Conference on Optical Elastography and Tissue Biomechanics , Canada , 1/02/14 . https://doi.org/10.1117/12.2039973 | en |
dc.identifier.citation | conference | en |
dc.identifier.issn | 0277-786X | |
dc.identifier.other | ORCID: /0000-0003-3422-3387/work/34730431 | |
dc.identifier.uri | https://hdl.handle.net/10023/5420 | |
dc.description.abstract | Shear stress has been recognized as one of the biophysical methods by which to permeabilize plasma membranes of cells. In particular, high pressure transient hydrodynamic flows created by laser-induced cavitation have been shown to lead to the uptake of fluorophores and plasmid DNA. While the mechanism and dynamics of cavitation have been extensively studied using a variety of time-resolved imaging techniques, the cellular response to the cavitation bubble and cavitation induced transient hydrodynamic flows has never been shown in detail. We use time-resolved quantitative phase microscopy to study cellular response to laser-induced cavitation bubbles. Laser-induced breakdown of an optically trapped polystyrene nanoparticle (500 nm in diameter) irradiated with a single nanosecond laser pulse at 532 nm creates transient shear stress to surrounding cells without causing cell lysis. A bi-directional transient displacement of cytoplasm is observed during expansion and collapse of the cavitation bubble. In some cases, cell deformation is only observable at the microsecond time scale without any permanent change in cell shape or optical thickness. On a time scale of seconds, the cellular response to shear stress and cytoplasm deformation typically leads to retraction of the cellular edge most exposed to the flow, rounding of the cell body and, in some cases, loss of cellular dry mass. These results give a new insight into the cellular response to laser-induced shear stress and related plasma membrane permeabilization. This study also demonstrates that laser-induced breakdown of an optically trapped nanoparticle offers localized cavitation (70 pm in diameter), which interacts with a single cell. | |
dc.format.extent | 7 | |
dc.format.extent | 1844993 | |
dc.language.iso | eng | |
dc.publisher | SPIE | |
dc.relation.ispartof | Optical Elastography and Tissue Biomechanics | en |
dc.relation.ispartofseries | Proceedings of SPIE | en |
dc.subject | Digital holography | en |
dc.subject | Time-resolved imaging | en |
dc.subject | Laser-induced breakdown | en |
dc.subject | Optical trapping | en |
dc.subject | Cell transfection | en |
dc.subject | Molecular delivery | en |
dc.subject | Laser | en |
dc.subject | Lyis | en |
dc.subject | Mechanisms | en |
dc.subject | Ablation | en |
dc.subject | Dynamics | en |
dc.subject | QC Physics | en |
dc.subject | QD Chemistry | en |
dc.subject | R Medicine (General) | en |
dc.subject.lcc | QC | en |
dc.subject.lcc | QD | en |
dc.subject.lcc | R1 | en |
dc.title | Imaging the cellular response to transient shear stress using time-resolved digital holography | en |
dc.type | Conference item | en |
dc.contributor.institution | University of St Andrews. School of Physics and Astronomy | en |
dc.contributor.institution | University of St Andrews. School of Medicine | en |
dc.contributor.institution | University of St Andrews. School of Biology | en |
dc.contributor.institution | University of St Andrews. Institute of Behavioural and Neural Sciences | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1117/12.2039973 |
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