Why flower visitation is a poor proxy for pollination : measuring single-visit pollen deposition, with implications for pollination networks and conservation

Abstract

Summary The relative importance of specialized and generalized plant-pollinator relationships is contentious, yet analyses usually avoid direct measures of pollinator quality (effectiveness), citing difficulties in collecting such data in the field and so relying on visitation data alone. We demonstrate that single-visit deposition (SVD) of pollen on virgin stigmas is a practical measure of pollinator effectiveness, using 13 temperate and tropical plant species. For each flower the most effective pollinator measured from SVD was as predicted from its pollination syndrome based on traditional advertisement and reward traits. Overall, c. 40% of visitors were not effective pollinators (range 0–78% for different flowers); thus, flower–pollinator relationships are substantially more specialized than visitation alone can reveal. Analyses at species level are crucial, as significant variation in SVD occurred within both higher-level taxonomic groups (genus, family) and within functional groups. Other measures sometimes used to distinguish visitors from pollinators (visit duration, frequency, or feeding behaviour in flowers) did not prove to be suitable proxies. Distinguishing between ‘pollinators’ and ‘visitors’ is therefore crucial, and true ‘pollination networks’ should include SVD to reveal pollinator effectiveness (PE). Generating such networks, now underway, could avoid potential misinterpretations of the conservation values of flower visitors, and of possible extinction threats as modelled in existing networks.