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dc.contributor.authorFuszard, M.A.
dc.contributor.authorOw, S.Y.
dc.contributor.authorGan, C.S.
dc.contributor.authorNoirel, J.
dc.contributor.authorTernan, N.G.
dc.contributor.authorMcMullan, G.
dc.contributor.authorBiggs, C.A.
dc.contributor.authorReardon, K.F.
dc.contributor.authorWright, P.C.
dc.date.accessioned2014-08-26T16:31:00Z
dc.date.available2014-08-26T16:31:00Z
dc.date.issued2013-02-26
dc.identifier.citationFuszard , M A , Ow , S Y , Gan , C S , Noirel , J , Ternan , N G , McMullan , G , Biggs , C A , Reardon , K F & Wright , P C 2013 , ' The quantitative proteomic response of Synechocystis sp. PCC6803 to phosphate acclimation ' Aquatic Biosystems , vol. 9 , no. 1 . DOI: 10.1186/2046-9063-9-5en
dc.identifier.issn2046-9063
dc.identifier.otherPURE: 143505960
dc.identifier.otherPURE UUID: 747c1246-e800-406e-a38b-e0788bd0b12f
dc.identifier.otherScopus: 84875076480
dc.identifier.urihttp://hdl.handle.net/10023/5242
dc.identifier.urihttp://www.scopus.com/inward/record.url?eid=2-s2.0-84875076480&partnerID=8YFLogxKen
dc.identifier.urihttp://www.aquaticbiosystems.org/content/9/1/5/additionalen
dc.descriptionThe authors wish to acknowledge the provision of an EPSRC studentship, Advanced Research Fellowship for CAB (EP/E053556/01) and further EPSRC funding (GR/S84347/01 and EP/E036252/1).en
dc.description.abstractBackground: Inorganic phosphate (P) is a critical nutrient for all life and is periodically limiting in marine and freshwater provinces, yet little is understood how organisms acclimate to fluctuations in Pi within their environment. To investigate whole cell adaptation, we grew Synechocystis sp. PCC6803, a model freshwater cyanobacterium, in 3%, and 0.3% inorganic phosphate (Pi) media. The cells were allowed to acclimate over 60 days, and cells were harvested for quantitative high throughput mass spectrometry-based proteomics using the iTRAQ™ labelling technology. Results: In total, 120 proteins were identified, and 52 proteins were considered differentially abundant compared to the control. Alkaline phosphatase (APase) activities correlated significantly (p <0.05) with observed relative PhoA abundances. PstS1 and PstS2 were both observed, yet PstS1 was not differentially more abundant than the control. Phycobilisome protein abundances appeared to be coordinated, and are significantly less abundant in 0.3% Pi than 3% Pi cultures. Also, the central metabolic cell function appears to have shifted towards the production of (NADPH) reducing energy and nucleotide sugars. Conclusions: This acclimation response bears strong similarity to the previously reported response to nitrogen deprivation within Synechocystis sp. PCC 6803. However, it also demonstrates some characteristics of desiccation stress, such as the regulation of fatty acids and increased abundance of rehydrin in the 3% Pi culture.en
dc.format.extent12en
dc.language.isoeng
dc.relation.ispartofAquatic Biosystemsen
dc.rights© 2013 Fuszard et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectCyanobacteriaen
dc.subjectiTRAQen
dc.subjectPhosphate acclimationen
dc.subjectPhycobilisomeen
dc.subjectPentose phosphate pathwayen
dc.subjectRibose sugarsen
dc.subjectSynechocystisen
dc.subjectQD Chemistryen
dc.subject.lccQDen
dc.titleThe quantitative proteomic response of Synechocystis sp. PCC6803 to phosphate acclimationen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Chemistryen
dc.identifier.doihttps://doi.org/10.1186/2046-9063-9-5
dc.description.statusPeer revieweden


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