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dc.contributor.authorPurohit, P
dc.contributor.authorPerez-Branguli , F
dc.contributor.authorProts, I
dc.contributor.authorBorger, Eva
dc.contributor.authorGunn-Moore, Frank J
dc.contributor.authorWelzel, O
dc.contributor.authorLoy, K
dc.contributor.authorWenzel, EM
dc.contributor.authorGromer, TW
dc.contributor.authorBrachs, S
dc.contributor.authorHolzer, M
dc.contributor.authorBuslei, R
dc.contributor.authorFritsch, K
dc.contributor.authorRegensburger, M
dc.contributor.authorBohm, KJ
dc.contributor.authorWinner, B
dc.contributor.authorMielenz, D
dc.date.accessioned2014-08-19T11:01:06Z
dc.date.available2014-08-19T11:01:06Z
dc.date.issued2014-08-18
dc.identifier.citationPurohit , P , Perez-Branguli , F , Prots , I , Borger , E , Gunn-Moore , F J , Welzel , O , Loy , K , Wenzel , EM , Gromer , TW , Brachs , S , Holzer , M , Buslei , R , Fritsch , K , Regensburger , M , Bohm , KJ , Winner , B & Mielenz , D 2014 , ' The Ca2+ sensor protein Swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neurons ' , PLoS One , vol. 9 , no. 8 , e103976 . https://doi.org/10.1371/journal.pone.0103976en
dc.identifier.issn1932-6203
dc.identifier.otherPURE: 136465420
dc.identifier.otherPURE UUID: 6beee6b4-e76d-46b9-aa49-68dfbf150a72
dc.identifier.otherScopus: 84915821571
dc.identifier.otherORCID: /0000-0003-3422-3387/work/34730427
dc.identifier.otherORCID: /0000-0003-4965-2969/work/30767001
dc.identifier.otherWOS: 000341302700020
dc.identifier.urihttp://hdl.handle.net/10023/5194
dc.descriptionThis work was supported by grants from the German Science Foundation (Deutsche Forschungsgemeinschaft, DFG; FOR832, to DM), the German Federal Ministry of Education and Research (01GQ113; to BW), the Bavarian Ministry of Sciences, Research and the Arts in the framework of the Bavarian Molecular Biosystems Reseach Network, the Interdisciplinary Center for Clinical Research (IZKF, Universitatsklinikum Erlangen; E8, to DM; NIII, to BW; Lab rotation to MR), the ELAN Fonds (Universitatsklinikum Erlangen; 11.08.19.1, to IP), and the Alzheimer’s Research UK (EB, FGM).en
dc.description.abstractSwiprosin-1/EFhd2 (EFhd2) is a cytoskeletal Ca2+ sensor protein strongly expressed in the brain. It has been shown to interact with mutant tau, which can promote neurodegeneration, but nothing is known about the physiological function of EFhd2 in the nervous system. To elucidate this question, we analyzed EFhd2-/-/lacZ reporter mice and showed that lacZ was strongly expressed in the cortex, the dentate gyrus, the CA1 and CA2 regions of the hippocampus, the thalamus, and the olfactory bulb. Immunohistochemistry and western blotting confirmed this pattern and revealed expression of EFhd2 during neuronal maturation. In cortical neurons, EFhd2 was detected in neurites marked by MAP2 and co-localized with preand post-synaptic markers. Approximately one third of EFhd2 associated with a biochemically isolated synaptosome preparation. There, EFhd2 was mostly confined to the cytosolic and plasma membrane fractions. Both synaptic endocytosis and exocytosis in primary hippocampal EFhd2-/- neurons were unaltered but transport of synaptophysin-GFP containing vesicles was enhanced in EFhd2-/- primary hippocampal neurons, and notably, EFhd2 inhibited kinesin mediated microtubule gliding. Therefore, we found that EFhd2 is a neuronal protein that interferes with kinesin-mediated transport.
dc.format.extent13
dc.language.isoeng
dc.relation.ispartofPLoS Oneen
dc.rights© 2014 Purohit et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.subjectQH301 Biologyen
dc.subject.lccQH301en
dc.titleThe Ca2+ sensor protein Swiprosin-1/EFhd2 is present in neurites and involved in kinesin-mediated transport in neuronsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Biologyen
dc.contributor.institutionUniversity of St Andrews.Institute of Behavioural and Neural Sciencesen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0103976
dc.description.statusPeer revieweden


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