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Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9
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dc.contributor.author | Sidik, Saima M. | |
dc.contributor.author | Hackett, Caroline G. | |
dc.contributor.author | Tran, Fanny | |
dc.contributor.author | Westwood, Nicholas J. | |
dc.contributor.author | Lourido, Sebastian | |
dc.date.accessioned | 2014-08-11T16:01:01Z | |
dc.date.available | 2014-08-11T16:01:01Z | |
dc.date.issued | 2014-06-27 | |
dc.identifier | 139613000 | |
dc.identifier | 5f102462-c325-4f8f-81a2-8a9f6b1d5731 | |
dc.identifier | 000338512200032 | |
dc.identifier | 84903544109 | |
dc.identifier | 000338512200032 | |
dc.identifier.citation | Sidik , S M , Hackett , C G , Tran , F , Westwood , N J & Lourido , S 2014 , ' Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9 ' , PLoS One , vol. 9 , no. 6 , e100450 . https://doi.org/10.1371/journal.pone.0100450 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.other | ORCID: /0000-0003-0630-0138/work/56424171 | |
dc.identifier.uri | https://hdl.handle.net/10023/5110 | |
dc.description | This work was supported in part by NIH grant 1DP5OD017892 to S.L | en |
dc.description.abstract | Toxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the T. gondii genome remains labor intensive, and is often restricted to lab-adapted strains or lines carrying mutations that enable selection. Here, we use the RNA-guided Cas9 nuclease to efficiently generate knockouts without selection, and to introduce point mutations and epitope tags into the T. gondii genome. These methods will streamline the functional analysis of parasite genes and enable high-throughput engineering of their genomes. | |
dc.format.extent | 8 | |
dc.format.extent | 1552210 | |
dc.language.iso | eng | |
dc.relation.ispartof | PLoS One | en |
dc.subject | Invasion | en |
dc.subject | Systems | en |
dc.subject | Marker | en |
dc.subject | Genes | en |
dc.subject | QD Chemistry | en |
dc.subject | SDG 3 - Good Health and Well-being | en |
dc.subject.lcc | QD | en |
dc.title | Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9 | en |
dc.type | Journal article | en |
dc.contributor.institution | University of St Andrews. School of Chemistry | en |
dc.contributor.institution | University of St Andrews. EaSTCHEM | en |
dc.contributor.institution | University of St Andrews. Biomedical Sciences Research Complex | en |
dc.identifier.doi | 10.1371/journal.pone.0100450 | |
dc.description.status | Peer reviewed | en |
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