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dc.contributor.authorPrystowsky, Michael
dc.contributor.authorFeeney, Katherine
dc.contributor.authorKawachi, Nicole
dc.contributor.authorMontagna, Cristina
dc.contributor.authorWillmott, Michelle
dc.contributor.authorWasson, Christopher
dc.contributor.authorAntkowiak, Maciej
dc.contributor.authorLoudig, Olivier
dc.contributor.authorParish, Joanna
dc.identifier.citationPrystowsky , M , Feeney , K , Kawachi , N , Montagna , C , Willmott , M , Wasson , C , Antkowiak , M , Loudig , O & Parish , J 2013 , ' Inhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G(2) delay and mitotic defects ' , Scientific Reports , vol. 3 , e2640 .
dc.identifier.otherPURE: 131088168
dc.identifier.otherPURE UUID: bc933c41-2f3b-4472-a31f-9ce713c420bd
dc.identifier.otherWOS: 000324229300003
dc.identifier.otherScopus: 84903062128
dc.descriptionWork by JP is funded by a Royal Society University Research Fellowship award. Experiments carried out by MBP were supported by the Department of Pathology, Albert Einstein College of Medicine / Montefiore Medical Center.en
dc.description.abstractThe development of clinically useful histone deacetylase inhibitors has expanded greatly. In a preclinical study, we showed that panobinostat (LBH589) inhibits cell cycle progression of human head and neck squamous cell carcinoma (HNSCC) cell lines at G(2)/M and an associated decrease in expression of particular genes required for passage through G(2) and mitosis. In this study we sought to analyse the mechanistic underpinnings of panobinostat-induced growth arrest. HNSCC cell lines were synchronised and progression through mitosis monitored. We demonstrate that panobinostat causes a marked G(2) delay and mitotic defects. A loss of G(2)-specific Plk1 and Cyclin B1 expression and co-incident increase in p21(Waf1/Cip1) expression is also shown. Furthermore, we show a significant loss of E2F1 recruitment to the promoters of these genes in response to panobinostat treatment. These data provide mechanistic evidence of panobinostat-induced cell cycle arrest and highlight its potential as a chemotherapeutic agent for HNSCC.
dc.relation.ispartofScientific Reportsen
dc.rights© 2013 The authors. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit
dc.subjectHistone deacetylase inhibitoren
dc.subjectCarcinoma-cell linesen
dc.subjectCancer cellsen
dc.subjectR Medicineen
dc.titleInhibition of Plk1 and Cyclin B1 expression results in panobinostat-induced G(2) delay and mitotic defectsen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews. School of Medicineen
dc.description.statusPeer revieweden

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