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dc.contributor.authorKos, Veronica N.
dc.contributor.authorDesjardins, Christopher A.
dc.contributor.authorGriggs, Allison
dc.contributor.authorCerqueira, Gustavo
dc.contributor.authorVan Tonder, Andries
dc.contributor.authorHolden, Matthew T. G.
dc.contributor.authorGodfrey, Paul
dc.contributor.authorPalmer, Kelli L.
dc.contributor.authorBodi, Kip
dc.contributor.authorMongodin, Emmanuel F.
dc.contributor.authorWortman, Jennifer
dc.contributor.authorFeldgarden, Michael
dc.contributor.authorLawley, Trevor
dc.contributor.authorGill, Steven R.
dc.contributor.authorHaas, Brian J.
dc.contributor.authorBirren, Bruce
dc.contributor.authorGilmore, Michael S.
dc.identifier.citationKos , V N , Desjardins , C A , Griggs , A , Cerqueira , G , Van Tonder , A , Holden , M T G , Godfrey , P , Palmer , K L , Bodi , K , Mongodin , E F , Wortman , J , Feldgarden , M , Lawley , T , Gill , S R , Haas , B J , Birren , B & Gilmore , M S 2012 , ' Comparative genomics of vancomycin-resistant Staphylococcus aureus strains and their positions within the clade most commonly associated with methicillin-resistant S. aureus hospital-acquired infection in the United States ' , mBio , vol. 3 , no. 3 , e00112-12 .
dc.identifier.otherPURE: 91753103
dc.identifier.otherPURE UUID: 42497b23-defa-4c46-95fb-2e027ef7afa3
dc.identifier.otherWOS: 000308587600011
dc.identifier.otherScopus: 84863442671
dc.identifier.otherORCID: /0000-0002-4958-2166/work/60196460
dc.descriptionThis research was supported in part with federal funds from the NIAID/NIH/DHHS, including the Harvard-wide Program on Antibiotic Resistance (NIH grant AI083214), NIH grant EY017381 (M.S.G.), contract HHSN27220090018C, and the NARSA program supported under NIAID/NIH contract HHSN272200700055C.en
dc.description.abstractMethicillin-resistant Staphylococcus aureus (MRSA) strains are leading causes of hospital-acquired infections in the United States, and clonal cluster 5 (CC5) is the predominant lineage responsible for these infections. Since 2002, there have been 12 cases of vancomycin-resistant S. aureus (VRSA) infection in the United States-all CC5 strains. To understand this genetic background and what distinguishes it from other lineages, we generated and analyzed high-quality draft genome sequences for all available VRSA strains. Sequence comparisons show unambiguously that each strain independently acquired Tn1546 and that all VRSA strains last shared a common ancestor over 50 years ago, well before the occurrence of vancomycin resistance in this species. In contrast to existing hypotheses on what predisposes this lineage to acquire Tn1546, the barrier posed by restriction systems appears to be intact in most VRSA strains. However, VRSA (and other CC5) strains were found to possess a constellation of traits that appears to be optimized for proliferation in precisely the types of polymicrobic infection where transfer could occur. They lack a bacteriocin operon that would be predicted to limit the occurrence of non-CC5 strains in mixed infection and harbor a cluster of unique superantigens and lipoproteins to confound host immunity. A frameshift in dprA, which in other microbes influences uptake of foreign DNA, may also make this lineage conducive to foreign DNA acquisition. IMPORTANCE Invasive methicillin-resistant Staphylococcus aureus (MRSA) infection now ranks among the leading causes of death in the United States. Vancomycin is a key last-line bactericidal drug for treating these infections. However, since 2002, vancomycin resistance has entered this species. Of the now 12 cases of vancomycin-resistant S. aureus (VRSA), each was believed to represent a new acquisition of the vancomycin-resistant transposon Tn1546 from enterococcal donors. All acquisitions of Tn1546 so far have occurred in MRSA strains of the clonal cluster 5 genetic background, the most common hospital lineage causing hospital-acquired MRSA infection. To understand the nature of these strains, we determined and examined the nucleotide sequences of the genomes of all available VRSA. Genome comparison identified candidate features that position strains of this lineage well for acquiring resistance to antibiotics in mixed infection.
dc.rightsCopyright © 2012 Kos et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.subjectHorizontal gene-transferen
dc.subjectNatural transformationen
dc.subjectPheromone cad1en
dc.subjectQR Microbiologyen
dc.titleComparative genomics of vancomycin-resistant Staphylococcus aureus strains and their positions within the clade most commonly associated with methicillin-resistant S. aureus hospital-acquired infection in the United Statesen
dc.typeJournal articleen
dc.description.versionPublisher PDFen
dc.contributor.institutionUniversity of St Andrews.School of Medicineen
dc.contributor.institutionUniversity of St Andrews.Infection Groupen
dc.contributor.institutionUniversity of St Andrews.Biomedical Sciences Research Complexen
dc.description.statusPeer revieweden

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